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验证数据展示

  • WB analysis of HeLa using 19838-1-AP

    WB analysis of HeLa using 19838-1-AP

    WB result of TMX2 antibody (19838-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMX2 transfected HeLa cells.

  • WB analysis using 19838-1-AP

    WB analysis using 19838-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19838-1-AP (TMX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 19838-1-AP

    IHC staining of human kidney using 19838-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19838-1-AP (TMX2 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 19838-1-AP

    IHC staining of human kidney using 19838-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19838-1-AP (TMX2 antibody) at dilution of 1:200 (under 40x lens).

  • Featured Product
  • KD/KO验证

TMX2 Polyclonal antibody

TMX2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19838-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CGI 31, My009, PDIA12, PIG26, PSEC0045, TMX2, TXNDC14, UNQ237/PRO270

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue, HeLa cells, mouse placenta tissue, mouse testis tissue, rat tissue
Positive IHC detected inhuman kidney tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19838-1-AP targets TMX2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13828

Product name: Recombinant human TMX2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 130-296 aa of BC000666

Sequence: PLYMGPEYIKYFNDKTIDEELERDKRVTWIVEFFANWSNDCQSFAPIYADLSLKYNCTGLNFGKVDVGRYTDVSTRYKVSTSPLTKQLPTLILFQGGKEAMRRPQIDKKGRAVSWTFSEENVIREFNLNELYQRAKKLSKAGDNIPEEQPVASTPTTVSDGENKKDK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thioredoxin-related transmembrane protein 2
别名 CGI 31, My009, PDIA12, PIG26, PSEC0045, TMX2, TXNDC14, UNQ237/PRO270
计算分子量 296 aa, 34 kDa
观测分子量 30 kDa, 34 kDa
GenBank蛋白编号BC000666
基因名称 TMX2
Gene ID (NCBI) 51075
RRIDAB_10642435
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y320
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thioredoxin-related transmembrane proteins (TMXs) are protein disulfide isomerase (PDI) family members and possess a transmembrane region. TMX2 cDNA was first isolated in 2003, and TMX2 mRNA has been found in a variety of human tissues, with the highest levels in the heart, brain, liver, kidney, and pancreas.

实验方案

Product Specific Protocols
WB protocol for TMX2 antibody 19838-1-APDownload protocol
IHC protocol for TMX2 antibody 19838-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols