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  • KD/KO Validated

TMPO/LAP2 Polyclonal antibody

TMPO/LAP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 14651-1-AP

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Synonyms

CMD1T, LAP2, LEMD4, PRO0868, Splenin Thymopentin, thymopoietin, Thymopoietin isoform alpha, TMPO, TMPO/LAP2, TP, TP alpha, TP5, TPRP isoform alpha



经过测试的应用

Positive WB detected inHEK-293 cells, HSC-T6 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue, mouse lung tissue, human liver cancer tissue, mouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TMPO Ready-To-Use IHC Kit. TMPO primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14651-1-AP targets TMPO/LAP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited ApplicationsIF, WB
Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, bovine
Immunogen TMPO/LAP2 fusion protein Ag6247 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thymopoietin
Synonyms CMD1T, LAP2, LEMD4, PRO0868, Splenin Thymopentin, thymopoietin, Thymopoietin isoform alpha, TMPO, TMPO/LAP2, TP, TP alpha, TP5, TPRP isoform alpha
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 75 kDa, 51 kDa and 39 kDa
GenBank Accession NumberBC053675
Gene Symbol TMPO
Gene ID (NCBI) 7112
RRIDAB_2205440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42167
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).

实验方案

Product Specific Protocols
WB protocol for TMPO/LAP2 antibody 14651-1-APDownload protocol
IHC protocol for TMPO/LAP2 antibody 14651-1-APDownload protocol
IF protocol for TMPO/LAP2 antibody 14651-1-APDownload protocol
IP protocol for TMPO/LAP2 antibody 14651-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Nat Commun

The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus.

Authors - Ying Zhang
humanIF

J Cell Biol

A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodies.

Authors - Tino Pleiner
humanWB

Sci Rep

MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells.

Authors - Yuhua Fu
bovineWB

J Dairy Sci

Anti-inflammation of epicatechin mediated by TMEM35A and TMPO in bovine mammary epithelial cell line cells and mouse mammary gland.

Authors - Xiao Ma
  • KO Validated
humanWB

World J Surg Oncol

Depletion of thymopoietin inhibits proliferation and induces cell cycle arrest/apoptosis in glioblastoma cells.

Authors - Lin Zhang
  • KD Validated
mouseIF

Theranostics

Progerin accumulation in nucleus pulposus cells impairs mitochondrial function and induces intervertebral disc degeneration and therapeutic effects of sulforaphane.

Authors - Xiaolong Xu