验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue, rat kidney tissue, Jurkat cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
产品信息
16621-1-AP targets TMLHE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rabbit |
| 免疫原 |
CatNo: Ag9919 Product name: Recombinant human TMLHE protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-376 aa of BC025269 Sequence: MWYHRLSHLHSRLQDLLKGGVIYPALPQPNFKSLLPLAVHWHHTASKSLTCAWQQHEDHFELKYANTVMRFDYVWLRDHCRSASCYNSKTHQRSLDTASVDLCIKPKTIRLDETTLFFTWPDGHVTKYDLNWLVKNSYEGQKQKVIQPRILWNAEIYQQAQVPSVDCQSFLETNEGLKKFLQNFLLYGIAFVENVPPTQEHTEKLAERISLIRETIYGRMWYFTSDFSRGDTAYTKLALDRHTDTTYFQEPCGIQVFHCLKHEGTGGRTLLVDGFYAAEQVLQKAPEEFELLSKVPLKHEYIEDVGECHNHMIGIGPVLNIYPWNKELYLIRLFKEKQNTVNRQWNSSLQCDIPERILTYRHFVSGTSIEHRGSLI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | trimethyllysine hydroxylase, epsilon |
| 别名 | BBOX2, EC:1.14.11.8, Epsilon-trimethyllysine 2-oxoglutarate dioxygenase, Epsilon-trimethyllysine hydroxylase, TML-alpha-ketoglutarate dioxygenase |
| 计算分子量 | 376 aa, 44 kDa |
| 观测分子量 | 44 kDa |
| GenBank蛋白编号 | BC025269 |
| 基因名称 | TMLHE |
| Gene ID (NCBI) | 55217 |
| RRID | AB_2205303 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NVH6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Trimethyllysine dioxygenase (TMLHE) encodes the first enzyme in carnitine biosynthesis, N6-trimethyllysine dioxygenase (TMLD) and enzyme deficiency was suggested to be a risk factor for ASD.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TMLHE antibody 16621-1-AP | Download protocol |
| IHC protocol for TMLHE antibody 16621-1-AP | Download protocol |
| WB protocol for TMLHE antibody 16621-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Discov Identification of BBOX1 as a Therapeutic Target in Triple-Negative Breast Cancer.
| ||
Cell Rep Inborn Errors of Long-Chain Fatty Acid β-Oxidation Link Neural Stem Cell Self-Renewal to Autism.
| ||
Cell Chem Biol Controlled lipid β-oxidation and carnitine biosynthesis by a vitamin D metabolite. | ||
bioRxiv αKG-mediated carnitine synthesis promotes homologous recombination via histone acetylation | ||
Nat Commun BBOX1 restrains TBK1-mTORC1 oncogenic signaling in clear cell renal cell carcinoma |
