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验证数据展示

  • WB analysis of A2780 using 60195-1-Ig

    WB analysis of A2780 using 60195-1-Ig

    A2780 cells were subjected to SDS PAGE followed by western blot with 60195-1-Ig (TMEM70 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 60195-1-Ig

    IHC staining of human liver using 60195-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 60195-1-Ig(TMEM70 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 60195-1-Ig

    IHC staining of human liver using 60195-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 60195-1-Ig(TMEM70 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of HeLa using 60195-1-Ig

    WB analysis of HeLa using 60195-1-Ig

    WB result of TMEM70 antibody (60195-1-Ig; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM70 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

TMEM70 Monoclonal antibody

TMEM70 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60195-1-Ig

产品说明书

CloneNo. 2C11C3
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, ELISA

别名

TMEM70, transmembrane protein 70

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA2780 cells, HeLa cells
Positive IHC detected inhuman liver tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

60195-1-Ig targets TMEM70 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 TMEM70 fusion protein Ag16947 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 transmembrane protein 70
别名 TMEM70, transmembrane protein 70
计算分子量 260 aa, 29 kDa
观测分子量 18 kDa
GenBank蛋白编号BC002748
基因名称 TMEM70
Gene ID (NCBI) 54968
RRIDAB_10896316
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BUB7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM70 belongs to the TMEM70 family. It is involved in biogenesis of mitochondrial ATP synthase. Defects in TMEM70 are a cause of mitochondrial encephalocardiomyopathy neonatal due to ATP synthase deficiency (MT-ATPSD). A publication (PMID:21147908) identified TMEM70 gene defect as a pan-ethnic disorder and further redefined it as the most common cause of nuclear-origin ATP synthase deficiency.

实验方案

Product Specific Protocols
WB protocol for TMEM70 antibody 60195-1-IgDownload protocol
IHC protocol for TMEM70 antibody 60195-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mitochondrion

Mitochondrial membrane assembly of TMEM70 protein.

Authors - Hana Kratochvílová
View Article