• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 21274-1-AP

    WB analysis using 21274-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21274-1-AP (TMEM64 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 21274-1-AP

    IHC staining of human prostate cancer using 21274-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21274-1-AP (TMEM64 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .

  • IHC staining of human prostate cancer using 21274-1-AP

    IHC staining of human prostate cancer using 21274-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21274-1-AP (TMEM64 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

TMEM64 Polyclonal antibody

TMEM64 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21274-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Transmembrane protein 64

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21274-1-AP targets TMEM64 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TMEM64 fusion protein Ag15753 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 64
别名 Transmembrane protein 64
计算分子量 380 aa, 40 kDa
观测分子量40-45 kDa
GenBank蛋白编号BC113828
基因名称 TMEM64
Gene ID (NCBI) 169200
RRIDAB_3085648
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6YI46
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tmem64 (transmembrane protein 64) is a positive modulator of osteoclast differentiation by SERCA2-dependent Ca2+ signaling (PMID: 23395171). Altered Levels of mRNAs for Calcium-Binding/Associated Proteins, Annexin A1, S100A4, and TMEM64 are associated with osteoporosis in peripheral blood mononuclear cells (PMID: 31814858). Tmem64 is involved in the metastatic progression of prostate cancer cells by regulating Wnt3a secretion (PMID: 31289498).

实验方案

Product Specific Protocols
WB protocol for TMEM64 antibody 21274-1-APDownload protocol
IHC protocol for TMEM64 antibody 21274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols