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验证数据展示

  • WB analysis using 21274-1-AP

    WB analysis using 21274-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21274-1-AP (TMEM64 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 21274-1-AP

    IHC staining of human prostate cancer using 21274-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21274-1-AP (TMEM64 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .

  • IHC staining of human prostate cancer using 21274-1-AP

    IHC staining of human prostate cancer using 21274-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21274-1-AP (TMEM64 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

TMEM64 Polyclonal antibody

TMEM64 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21274-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Transmembrane protein 64

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21274-1-AP targets TMEM64 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15753

Product name: Recombinant human TMEM64 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 301-380 aa of BC113828

Sequence: DVIAEQSVSGYFVFCLQIIISIGLMFYVVHRAQVELNAAIVACEMELKSSLVKGNQPNTSGSSFYNKRTLTFSGGGINVV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 64
别名 Transmembrane protein 64
计算分子量 380 aa, 40 kDa
观测分子量40-45 kDa
GenBank蛋白编号BC113828
基因名称 TMEM64
Gene ID (NCBI) 169200
RRIDAB_3085648
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6YI46
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tmem64 (transmembrane protein 64) is a positive modulator of osteoclast differentiation by SERCA2-dependent Ca2+ signaling (PMID: 23395171). Altered Levels of mRNAs for Calcium-Binding/Associated Proteins, Annexin A1, S100A4, and TMEM64 are associated with osteoporosis in peripheral blood mononuclear cells (PMID: 31814858). Tmem64 is involved in the metastatic progression of prostate cancer cells by regulating Wnt3a secretion (PMID: 31289498).

实验方案

Product Specific Protocols
WB protocol for TMEM64 antibody 21274-1-APDownload protocol
IHC protocol for TMEM64 antibody 21274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols