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验证数据展示

  • FC experiment of Transfected CHO-K1 using 98677-3-RR (same clone as 98677-3-PBS)

    FC experiment of Transfected CHO-K1 using 98677-3-RR (same clone as 98677-3-PBS)

    1x10^6 CHO-K1(left) or TMEM119-transfected CHO-K1(right) were surface stained with 0.25 ug Anti-Human TMEM119 Rabbit RecAb (98677-3-RR, Clone:251557E2) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98677-3-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 98677-3-RR (same clone as 98677-3-PBS)

    FC experiment of SH-SY5Y using 98677-3-RR (same clone as 98677-3-PBS)

    1x10^6 SH-SY5Y cells were stained with 0.25 ug Anti-Human TMEM119 Rabbit RecAb (98677-3-RR, Clone:251557E2) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98677-3-PBS in a different storage buffer formulation.

  • FC experiment of Transfected CHO-K1 using 98677-3-RR (same clone as 98677-3-PBS)

    FC experiment of Transfected CHO-K1 using 98677-3-RR (same clone as 98677-3-PBS)

    1x10^6 CHO-K1(left) or TMEM119-transfected CHO-K1(right) were surface stained with 0.25 ug Anti-Human TMEM119 Rabbit RecAb (98677-3-RR, Clone:251557E2) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98677-3-PBS in a different storage buffer formulation.

  • Affinity of 98677-3-RR

    Affinity of 98677-3-RR

    Biolayer interferometry (BLl) kinetic assays of 98677-3-RR against Human TMEM119 were performed. The affinity constant is 6.55 nM.

Anti-Human TMEM119 Rabbit Recombinant Antibody, PBS Only

TMEM119 Uni-rAb® Recombinant Antibody for FC
Cat No. 98677-3-PBS

产品说明书

CloneNo. 251557E2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

OBIF, Osteoblast induction factor, transmembrane protein 119, UNQ731/PRO1415

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98677-3-PBS targets TMEM119 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg1823

Product name: Recombinant human TMEM119 protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 26-96 aa of NM_181724

Sequence: RSVPLKATFLEDVAGSGEAEGSSASSPSLPPPWTPALSPTSMGPQPITLGGPSPPTNFLDGIVDFFRQYVM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane protein 119
别名 OBIF, Osteoblast induction factor, transmembrane protein 119, UNQ731/PRO1415
计算分子量29 kDa
GenBank蛋白编号NM_181724
基因名称 TMEM119
Gene ID (NCBI) 338773
RRIDAB_3746265
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ4V9L6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM119 (Transmembrane Protein 119), also known as OBIF (Osteoblast Induction Factor), is a type I transmembrane protein that serves as a highly specific marker for resident microglia in the central nervous system (CNS). TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788).

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