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验证数据展示

  • WB analysis using 67205-1-Ig (same clone as 67205-1-PBS)

    WB analysis using 67205-1-Ig (same clone as 67205-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67205-1-Ig (TMEM111 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 67205-1-Ig (same clone as 67205-1-PBS)

    IHC staining of mouse lung using 67205-1-Ig (same clone as 67205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67205-1-Ig (TMEM111 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 67205-1-Ig (same clone as 67205-1-PBS)

    IHC staining of mouse lung using 67205-1-Ig (same clone as 67205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67205-1-Ig (TMEM111 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67205-1-Ig (same clone as 67205-1-PBS)

    WB analysis of HEK-293 using 67205-1-Ig (same clone as 67205-1-PBS)

    WB result of TMEM111 antibody (67205-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM111 transfected HEK-293 cells. This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 67205-1-Ig (same clone as 67205-1-PBS)

    IF Staining of A431 using 67205-1-Ig (same clone as 67205-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using TMEM111 antibody (67205-1-Ig, Clone: 2F8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TMEM111 Monoclonal antibody, PBS Only

TMEM111 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67205-1-PBS

产品说明书

CloneNo. 2F8G5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EMC3, POB, TMEM111, transmembrane protein 111

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67205-1-PBS targets TMEM111 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 TMEM111 fusion protein Ag18365 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 transmembrane protein 111
别名 EMC3, POB, TMEM111, transmembrane protein 111
计算分子量 261 aa, 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC022807
基因名称 TMEM111
Gene ID (NCBI) 55831
RRIDAB_2882498
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9P0I2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM111,also names as EMC3, is part of the endoplasmic reticulum-associated secretory pathway (PMID: 19797678). TMEM111 was required for murine pulmonary surfactant synthesis and lung function at birth (PMID: 29083321). It can be detect a band of 30 kDa.