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验证数据展示

  • WB analysis using 67205-1-Ig (same clone as 67205-1-PBS)

    WB analysis using 67205-1-Ig (same clone as 67205-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67205-1-Ig (TMEM111 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 67205-1-Ig (same clone as 67205-1-PBS)

    IHC staining of mouse lung using 67205-1-Ig (same clone as 67205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67205-1-Ig (TMEM111 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 67205-1-Ig (same clone as 67205-1-PBS)

    IHC staining of mouse lung using 67205-1-Ig (same clone as 67205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67205-1-Ig (TMEM111 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67205-1-Ig (same clone as 67205-1-PBS)

    WB analysis of HEK-293 using 67205-1-Ig (same clone as 67205-1-PBS)

    WB result of TMEM111 antibody (67205-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM111 transfected HEK-293 cells. This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 67205-1-Ig (same clone as 67205-1-PBS)

    IF Staining of A431 using 67205-1-Ig (same clone as 67205-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using TMEM111 antibody (67205-1-Ig, Clone: 2F8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TMEM111 Monoclonal antibody, PBS Only

TMEM111 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67205-1-PBS

产品说明书

CloneNo. 2F8G5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EMC3, 2F8G5, POB

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67205-1-PBS targets TMEM111 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 TMEM111 fusion protein Ag18365 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 transmembrane protein 111
别名 EMC3, 2F8G5, POB
计算分子量 261 aa, 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC022807
基因名称 TMEM111
Gene ID (NCBI) 55831
RRIDAB_2882498
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9P0I2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM111,also names as EMC3, is part of the endoplasmic reticulum-associated secretory pathway (PMID: 19797678). TMEM111 was required for murine pulmonary surfactant synthesis and lung function at birth (PMID: 29083321). It can be detect a band of 30 kDa.