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验证数据展示

  • WB analysis using 24645-1-AP

    WB analysis using 24645-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24645-1-AP (TMEM111 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 24645-1-AP

    WB analysis of HEK-293 using 24645-1-AP

    WB result of TMEM111 antibody (24645-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM111 transfected HEK-293 cells.

  • IF Staining of A431 using 24645-1-AP

    IF Staining of A431 using 24645-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using TMEM111 antibody (24645-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 24645-1-AP

    IHC staining of human ovary cancer using 24645-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 24645-1-AP (TMEM111 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM111 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 24645-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 24645-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24645-1-AP (TMEM111 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM111 antibody included
  • Featured Product
  • KD/KO验证

TMEM111 Polyclonal antibody

TMEM111 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24645-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EMC3, POB

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HuH-7 cells, MDA-MB-453s cells, NIH/3T3 cells, mouse brain tissue, mouse stomach tissue, mouse testis tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy® TMEM111 Ready-To-Use IHC Kit. TMEM111 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24645-1-AP targets TMEM111 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18236

Product name: Recombinant human TMEM111 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 139-261 aa of BC022807

Sequence: KVPFPLTLRFKPMLQQGIELLTLDASWVSSASWYFLNVFGLRSIYSLILGQDNAADQSRMMQEQMTGAAMAMPADTNKAFKTEWEALELTDHQWALDDVEEELMAKDLHFEGMFKKELQTSIF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 111
别名 EMC3, POB
计算分子量 261 aa, 30 kDa
观测分子量30 kDa
GenBank蛋白编号BC022807
基因名称 TMEM111
Gene ID (NCBI) 55831
RRIDAB_3085744
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P0I2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM111,also names as EMC3, is part of the endoplasmic reticulum-associated secretory pathway (PMID: 19797678). TMEM111 was required for murine pulmonary surfactant synthesis and lung function at birth (PMID: 29083321). It can be detect a band of 30 kDa.

实验方案

Product Specific Protocols
IF protocol for TMEM111 antibody 24645-1-APDownload protocol
IHC protocol for TMEM111 antibody 24645-1-APDownload protocol
WB protocol for TMEM111 antibody 24645-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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