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验证数据展示

  • WB analysis using 11928-1-AP

    WB analysis using 11928-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11928-1-AP (TMEFF2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11928-1-AP

    IHC staining of human prostate cancer using 11928-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11928-1-AP (TMEFF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEFF2 antibody included
  • IHC staining of human prostate cancer using 11928-1-AP

    IHC staining of human prostate cancer using 11928-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11928-1-AP (TMEFF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEFF2 antibody included

TMEFF2 Polyclonal antibody

TMEFF2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11928-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

HPP1, TENB2, TMEFF2, Tomoregulin 2, TPEF, TR, TR 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HeLa cells, human brain tissue, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TMEFF2 Ready-To-Use IHC Kit. TMEFF2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11928-1-AP targets TMEFF2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag2609

Product name: Recombinant human TMEFF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-374 aa of BC008973

Sequence: MVLWESPRQCSSWTLCEGFCWLLLLPVMLLIVARPVKLAAFPTSLSDCQTPTGWNCSGYDDRENDLFLCDTNTCKFDGECLRIGDTVTCVCQFKCNNDYVPVCGSNGESYQNECYLRQAACKQQSEILVVSEGSCATDAGSGSGDGVHEGSGETSQKETSTCDICQFGAECDEDAEDVWCVCNIDCSQTNFNPLCASDGKSYDNACQIKEASCQKQEKIEVMSLGRCQDNTTTTTKSEDGHYARTDYAENANKLEESAREHHIPCPEHYNGFCMHGKCEHSINMQEPSCRCDAGYTGQHCEKKDYSVLYVVPGPVRFQYVLIAAVIGTIQIAVICVVVLCITRKCPRSNRIHRQKQNTGHYSSDNTTRASTRLI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein with EGF-like and two follistatin-like domains 2
别名 HPP1, TENB2, TMEFF2, Tomoregulin 2, TPEF, TR, TR 2
计算分子量 374 aa, 41 kDa
观测分子量 41 kDa
GenBank蛋白编号BC008973
基因名称 TMEFF2
Gene ID (NCBI) 23671
RRIDAB_2204477
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UIK5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMEFF2 antibody 11928-1-APDownload protocol
IHC protocol for TMEFF2 antibody 11928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Immunol

Gene expression profiling and real-time PCR analyses identify novel potential cancer-testis antigens in multiple myeloma.

Authors - Condomines Maud M
View Article
humanIHC

Cancer Cell Int

TMEFF2 promoter hypermethylation is an unfavorable prognostic marker in gliomas.

Authors - Sidi Xie
View Article