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验证数据展示

  • WB analysis using 21620-1-AP (same clone as 21620-1-PBS)

    WB analysis using 21620-1-AP (same clone as 21620-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 21620-1-AP (same clone as 21620-1-PBS)

    IP experiment of HepG2 using 21620-1-AP (same clone as 21620-1-PBS)

    IP result of anti-TMED9 (IP:21620-1-AP, 4ug; Detection:21620-1-AP 1:4000) with HepG2 cells lysate 1600 ug. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 21620-1-AP (same clone as 21620-1-PBS)

    IHC staining of human liver using 21620-1-AP (same clone as 21620-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21620-1-AP (TMED9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 21620-1-AP (same clone as 21620-1-PBS)

    IHC staining of human liver using 21620-1-AP (same clone as 21620-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21620-1-AP (TMED9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 21620-1-AP (same clone as 21620-1-PBS)

    IF Staining of HepG2 using 21620-1-AP (same clone as 21620-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TMED9 antibody (21620-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TMED9 Polyclonal antibody, PBS Only

TMED9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 21620-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Glycoprotein 25L2, GMP25, GP25L2, p24 family protein alpha-2, p24alpha2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21620-1-PBS targets TMED9 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag13813

Product name: Recombinant human TMED9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-224 aa of BC001123

Sequence: MAVELGVLLVRPRPGTGLGRVMRTLLLVLWLATRGSALYFHIGETEKKCFIEEIPDETMVIGNYRTQLYDKQREEYQPATPGLGMFVEVKDPEDKVILARQYGSEGRFTFTSHTPGEHQICLHSNSTKFSLFAGGMLRVHLDIQVGEHANDYAEIAAKDKLSELQLRVRQLVEQVEQIQKEQNYQRWREERFRQTSESTNQRVLWWSILQTLILVAIGVWQMRH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane emp24 protein transport domain containing 9
别名 Glycoprotein 25L2, GMP25, GP25L2, p24 family protein alpha-2, p24alpha2
计算分子量 235 aa, 27 kDa
观测分子量 24-27 kDa
GenBank蛋白编号BC001123
基因名称 TMED9
Gene ID (NCBI) 54732
RRIDAB_10858623
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BVK6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMED9 (also named as GMP25, GP25L2 or p25) is a member of the EMP24/GP25L family. TMED9 probably forms hetero-oligomeric complexes with TMED7 (p27), TMED2 (p24), and TMED10 (p23) (PMID: 10359607). Located in the endoplasmic reticulum and the Golgi, TMED9 appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway (PMID: 9472029; 10359607).

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