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验证数据展示

  • FC experiment of HeLa using CL594-67876

    FC experiment of HeLa using CL594-67876

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TMP21 (CL594-67876, Clone:3B11F9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated TMP21 Monoclonal antibody

TMP21 Monoclonal Antibody for FC (Intra)
Cat No. CL594-67876

产品说明书

CloneNo. 3B11F9

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

FC (Intra)

别名

TMED10, Protein TMED10, p24delta1, p24 family protein delta-1, p23

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67876-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67876 targets TMP21 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag7516

Product name: Recombinant human TMED10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 40-186 aa of BC001825

Sequence: SRKCLREEIHKDLLVTGAYEISDQSGGAGGLRSHLKITDSAGHILYSKEDATKGKFAFTTEDYDMFEVCFESKGTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRV

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 transmembrane emp24-like trafficking protein 10 (yeast)
别名 TMED10, Protein TMED10, p24delta1, p24 family protein delta-1, p23
计算分子量 25 kDa
观测分子量21 kDa
GenBank蛋白编号BC001825
基因名称 TMED10
Gene ID (NCBI) 10972
RRIDAB_2923929
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49755
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL594 TMP21 antibody CL594-67876Download protocol
Standard Protocols
Click here to view our Standard Protocols