验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, L02 cells, Calu-3 cells, MDA-MB-231 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86440-1-RR targets TMCO1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag26278 Product name: Recombinant human TMCO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 111-188 aa of NM_019026 Sequence: MGRVVAKLPFTPLSYIQGLSHRNLLGDDTTDCSFIFLYILCTMSIRQNIQKILGLAPSRAATKQAGGFLGPPPPSGKFS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | transmembrane and coiled-coil domains 1 |
| 别名 | HP10122, PCIA3, PNAS 136, RP11 466F5.7, TMCC4 |
| 计算分子量 | 21 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | NM_019026 |
| 基因名称 | TMCO1 |
| Gene ID (NCBI) | 54499 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UM00 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMCO1, also known as TMCC4, PNAS-10, and PNAS-136, is a 188 amino acid protein, which is widely expressed in adult and fetal tissues, with higher levels in thymus, prostate, testis and small intestine and lower levels in brain, placenta, lung and kidney (PubMed:10393320, PubMed:20018682). TMCO1 as a Calcium-selective channel is required to prevent calcium stores from overfilling, thereby playing a key role in calcium homeostasis (PubMed:27212239). In response to endoplasmic reticulum overloading, TMCO1, assembles into a homotetramer, forming a functional calcium-selective channel, regulating the calcium content in endoplasmic reticulum store (PubMed:27212239).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMCO1 antibody 86440-1-RR | Download protocol |
| WB protocol for TMCO1 antibody 86440-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
