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验证数据展示

  • FC experiment of human PBMCs using 98327-1-RR

    FC experiment of human PBMCs using 98327-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TLR4/CD284 Rabbit RecAb (98327-1-RR, Clone:242067C1) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Donkey Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 98327-1-RR

    FC experiment of human PBMCs using 98327-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TLR4/CD284 Rabbit RecAb (98327-1-RR, Clone: 242067C1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Donkey Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Anti-Human TLR4/CD284 Rabbit Recombinant Antibody

TLR4/CD284 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98327-1-RR

产品说明书

CloneNo. 242067C1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

TLR4, 242067C1, ARMD10, CD284, hToll

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98327-1-RR targets TLR4/CD284 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 toll-like receptor 4
别名 TLR4, 242067C1, ARMD10, CD284, hToll
计算分子量 96 kDa
GenBank蛋白编号NM_138554
基因名称 TLR4
Gene ID (NCBI) 7099
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00206
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.

实验方案

Product Specific Protocols
FC protocol for TLR4/CD284 antibody 98327-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols