验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | human monocyte-derived immature dendritic cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-SF98016 targets TLR3/CD283 in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag34044 Product name: Recombinant human TLR3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 725-904 aa of BC094737 Sequence: FEGWRISFYWNVSVHRVLGFKEIDRQTEQFEYAAYIIHAYKDKDWVWEHFSSMEKEDQSLKFCLEERDFEAGVFELEAIVNSIKRSRKIIFVITHHLLKDPLCKRFKVHHAVQQAIEQNLDSIILVFLEEIPDYKLNHALCLRRGMFKSHCILNWPVQKERIGAFRHKLQVALGSKNSVH 种属同源性预测 |
| 宿主/类型 | Rabbit / scFv |
| 抗体类别 | Recombinant |
| 产品类型 | Single-chain variable fragment antibody (scFv) |
| 全称 | toll-like receptor 3 |
| 别名 | TLR3, CD283, toll like receptor 3, Toll-like receptor 3 |
| 计算分子量 | 904 aa, 104 kDa |
| GenBank蛋白编号 | BC094737 |
| 基因名称 | TLR3 |
| Gene ID (NCBI) | 7098 |
| RRID | AB_3748938 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 激发激光 | Red Laser (633 nm) |
| 来源 | Anti-Human TLR3/CD283 scFv from clone 230445F1, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His tag at the C-terminus. |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin300, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 TLR3/CD283 antibody CL647-SF98016 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
