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  • Featured Product
  • KD/KO Validated

TLR2 Monoclonal antibody, PBS Only

TLR2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C1D3

Cat No : 66645-1-PBS

Print datasheet

Synonyms

CD282, TIL4, TLR2, toll like receptor 2

验证数据展示

  • WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

    WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

    WB result of TLR2 antibody (66645-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLR2 transfected THP-1 cells. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66645-1-Ig (same clone as 66645-1-PBS)

    IHC staining of human liver using 66645-1-Ig (same clone as 66645-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66645-1-Ig (TLR2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

    IF Staining of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR2 antibody (66645-1-Ig, Clone: 1C1D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

    WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66645-1-Ig (TLR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

产品信息

66645-1-PBS targets TLR2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF, IHC, Indirect ELISA
Tested Reactivity Human
Immunogen TLR2 fusion protein Ag25626 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name toll-like receptor 2
Synonyms CD282, TIL4, TLR2, toll like receptor 2
Calculated Molecular Weight 784 aa, 90 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC033756
Gene Symbol TLR2
Gene ID (NCBI) 7097
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60603
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

TLR2, also known as CD282, is a member of the Toll-like receptor (TLR) family. TLR receptors are type I integral transmembrane glycoproteins which play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs) and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).