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TLR2 Monoclonal antibody

TLR2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (2)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C1D3

Cat No : 66645-1-Ig

Print datasheet

Synonyms

CD282, TIL4, TLR2, toll like receptor 2



经过测试的应用

Positive WB detected inTHP-1 cells, U-937 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inTHP-1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66645-1-Ig targets TLR2 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity Human
Cited Reactivityhuman, pig
Immunogen TLR2 fusion protein Ag25626 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name toll-like receptor 2
Synonyms CD282, TIL4, TLR2, toll like receptor 2
Calculated Molecular Weight 784 aa, 90 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC033756
Gene Symbol TLR2
Gene ID (NCBI) 7097
RRIDAB_2882003
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60603
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLR2, also known as CD282, is a member of the Toll-like receptor (TLR) family. TLR receptors are type I integral transmembrane glycoproteins which play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs) and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).

实验方案

Product Specific Protocols
WB protocol for TLR2 antibody 66645-1-IgDownload protocol
IHC protocol for TLR2 antibody 66645-1-IgDownload protocol
IF protocol for TLR2 antibody 66645-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Clin Transl Med

Monocyte biomarkers define sargramostim treatment outcomes for Parkinson's disease.

Authors - Mai M Abdelmoaty
humanWB

Life Sci

The novel mechanism of human norovirus induced diarrhea: Activation of PKD2 caused by HuNoVs destroyed AQP3 expression through AP2γ in intestinal epithelial cells

Authors - Ling Huang
humanWB,IF

Microbes Infect

Corydalis Saxicola Bunting Total Alkaloid Eliminates Porphyromonas gingivalis strain 33277 Internalized into Macrophages by Inhibition of TLR2

Authors - Lan Yang
pigWB

Cells

Clostridium Butyricum ZJU-F1 Benefits the Intestinal Barrier Function and Immune Response Associated with Its Modulation of Gut Microbiota in Weaned Piglets.

Authors - Jie Fu
humanWB

Front Microbiol

PPARγ Ameliorates Mycobacterium tuberculosis H37Ra-Induced Foamy Macrophage Formation via the ABCG1-Dependent Cholesterol Efflux Pathway in THP-1 Macrophages.

Authors - Yutao Ye
humanWB

ACS Omega

Azilsartan Suppressed LPS-Induced Inflammation in U937 Macrophages through Suppressing Oxidative Stress and Inhibiting the TLR2/MyD88 Signal Pathway.

Authors - Qinglian Dong