验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, HEK-293T cells, MOLT-4 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85877-1-RR targets TKTL1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag4913 Product name: Recombinant human TKTL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC025382 Sequence: MADAEARAEFPEEARPDRGTLQVFQDMASRLRIHSIRATCSTSSGHPTSCSSSSEIMSVLFFYIMRYKQSDPENPDNDRFVLAKRLSFVDVATGWLGQGLGVACGMAYTGKYFDRASYRVFCLMSDGESSEGSVWEAMAFASYYSLDNLVATFDVNRLGHSGALPAEHCINIYQRRCEAFGWNTYVVDGRDVEALCQVFWQASQVKHKPTAVVAKTFKGRGTPSIEDAESWHAKPMPRERADAIIKLIESQIQTSRNLDPQPPIEDSPEVNITDVRMTSPPDYRVGDKIATRKACGLALA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | transketolase-like 1 |
| 别名 | EC:2.2.1.1, TK 2, TK2, TKR, TKT 2 |
| 计算分子量 | 65 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC025382 |
| 基因名称 | TKTL1 |
| Gene ID (NCBI) | 8277 |
| RRID | AB_3744439 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P51854 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transketolase-like 1 (TKTL1) is a gene from the transketolase family that in fetal human neocortex is preferentially expressed in the two classes of neuroprogenitors, the apical progenitors in the ventricular zone and the basal progenitors in the subventricular zone.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TKTL1 antibody 85877-1-RR | Download protocol |
| WB protocol for TKTL1 antibody 85877-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
