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验证数据展示

  • WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)

    WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67787-1-Ig (same clone as 67787-1-PBS)

    IHC staining of human colon cancer using 67787-1-Ig (same clone as 67787-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67787-1-Ig (TK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TK1 antibody included
  • IHC staining of human colon cancer using 67787-1-Ig (same clone as 67787-1-PBS)

    IHC staining of human colon cancer using 67787-1-Ig (same clone as 67787-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67787-1-Ig (TK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TK1 antibody included
  • WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)

    WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

  • WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)

    WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67787-1-Ig (same clone as 67787-1-PBS)

    IHC staining of human tonsillitis using 67787-1-Ig (same clone as 67787-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67787-1-Ig (TK1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TK1 antibody included
  • IF Staining of HCT 116 using 67787-1-Ig (same clone as 67787-1-PBS)

    IF Staining of HCT 116 using 67787-1-Ig (same clone as 67787-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using TK1 antibody (67787-1-Ig, Clone: 1B1C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

TK1 Monoclonal antibody, PBS Only

TK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67787-1-PBS

产品说明书

CloneNo. 1B1C2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Tk1b, Tk1a, TK 2, TK 1, EC:2.7.1.21

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TK1 Ready-To-Use IHC Kit. TK1 primary antibody included.

产品信息

67787-1-PBS targets TK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8265

Product name: Recombinant human TK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-234 aa of BC007986

Sequence: MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFFPDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAARKLFAPQQILQCSPAN

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 thymidine kinase 1, soluble
别名 Tk1b, Tk1a, TK 2, TK 1, EC:2.7.1.21
计算分子量 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC007986
基因名称 TK1
Gene ID (NCBI) 7083
RRIDAB_2918551
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04183
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264).

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