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验证数据展示

  • WB analysis using 11973-1-AP (same clone as 11973-1-PBS)

    WB analysis using 11973-1-AP (same clone as 11973-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11973-1-AP (TIMM13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11973-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11973-1-AP (same clone as 11973-1-PBS)

    IF Staining of HepG2 using 11973-1-AP (same clone as 11973-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11973-1-AP (TIMM13 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11973-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 11973-1-AP (same clone as 11973-1-PBS)

    IHC staining of human liver cancer using 11973-1-AP (same clone as 11973-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11973-1-AP (TIMM13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11973-1-PBS in a different storage buffer formulation.

TIMM13 Polyclonal antibody, PBS Only

TIMM13 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11973-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11973-1-PBS targets TIMM13 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2596

Product name: Recombinant human TIMM13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-95 aa of BC008607

Sequence: MEGGFGSDFGGSGSGKLDPGLIMEQVKVQIAVANAQELLQRMTDKCFRKCIGKPGGSLDNSEQKCIAMCMDRYMDAWNTVSRAYNSRLQRERANM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 translocase of inner mitochondrial membrane 13 homolog (yeast)
别名
计算分子量 95 aa, 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC008607
基因名称 TIMM13
Gene ID (NCBI) 26517
RRIDAB_2287228
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5L4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIMM13 gene, also known as TIM13, TIM13B, TIM M13A or TIMM13B, encodes mitochondrial import inner membrane translocase subunit Tim13 belonging to the small Tim family. TIM13 functions as mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Proteins of the intermembrane space (IMS) of mitochondria are typically synthesized without presequences. TIM13 contains four conserved cysteine residues that bind a zinc ion as cofactor. Import of TIM13 did not depend on the membrane potential or ATP hydrolysis. Upon import into mitochondria TIM13 adopted a stably folded conformation in the IMS.

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