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验证数据展示

  • WB analysis using 67022-1-Ig (same clone as 67022-1-PBS)

    WB analysis using 67022-1-Ig (same clone as 67022-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 67022-1-Ig (same clone as 67022-1-PBS)

    FC experiment of HEK-293 using 67022-1-Ig (same clone as 67022-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human TIMELESS (67022-1-Ig, Clone:1B6G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TIMELESS Monoclonal antibody, PBS Only

TIMELESS Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 67022-1-PBS

产品说明书

CloneNo. 1B6G3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Indirect ELISA

别名

TIMELESS1, timeless homolog (Drosophila), TIM1, TIM, Protein timeless homolog

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67022-1-PBS targets TIMELESS in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TIMELESS fusion protein Ag6283 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 timeless homolog (Drosophila)
别名 TIMELESS1, timeless homolog (Drosophila), TIM1, TIM, Protein timeless homolog
计算分子量 139 kDa
观测分子量 140 kDa
GenBank蛋白编号BC050557
基因名称 TIMELESS
Gene ID (NCBI) 8914
RRIDAB_2882337
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UNS1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.