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验证数据展示

  • WB analysis using 82836-1-RR (same clone as 82836-1-PBS)

    WB analysis using 82836-1-RR (same clone as 82836-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82836-1-RR (TIM3 antibody) at dilution of 1:3500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82836-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 82836-1-RR (same clone as 82836-1-PBS)

    WB analysis of PC-3 using 82836-1-RR (same clone as 82836-1-PBS)

    WB result of TIM3 antibody (82836-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIM3 transfected PC-3 cells. This data was developed using the same antibody clone with 82836-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82836-1-RR

    Affinity and Kinetic Characterization of 82836-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82836-1-RR against Human TIM3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证
    • {{ptg:ProductFeaturesInfo}}

TIM3 Recombinant monoclonal antibody, PBS Only

TIM3 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 82836-1-PBS

产品说明书

CloneNo. 5A8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82836-1-PBS targets TIM3 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2459

Product name: Recombinant human HAVCR2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-142 aa of BC020843

Sequence: MFSHLPFDCVLLLLLLLLTRSSEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPGEWTFACHLYE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hepatitis A virus cellular receptor 2
别名 T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366
计算分子量 301 aa, 33 kDa
观测分子量70 kDa
GenBank蛋白编号BC020843
基因名称 TIM3
Gene ID (NCBI) 84868
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8TDQ0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).