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TIM3 Recombinant antibody

TIM3 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

5A8

Cat No : 82836-1-RR

Print datasheet

Synonyms

FLJ14428, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIMD 3, TIMD3

验证数据展示

  • WB analysis using 82836-1-RR

    WB analysis using 82836-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82836-1-RR (TIM3 antibody) at dilution of 1:3500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPC-3 cells, THP-1 cells, Daudi cells, RAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:7000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82836-1-RR targets TIM3 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, ELISA
Tested Reactivity Human, Mouse
Immunogen TIM3 fusion protein Ag2459 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hepatitis A virus cellular receptor 2
Synonyms FLJ14428, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIMD 3, TIMD3
Calculated Molecular Weight 301 aa, 33 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC020843
Gene Symbol HAVCR2
Gene ID (NCBI) 84868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TDQ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).

实验方案

Product Specific Protocols
WB protocol for TIM3 antibody 82836-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols