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验证数据展示

  • WB analysis using 14927-1-AP (same clone as 14927-1-PBS)

    WB analysis using 14927-1-AP (same clone as 14927-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14927-1-AP (TIM22 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 14927-1-AP (same clone as 14927-1-PBS)

    IHC staining of human liver using 14927-1-AP (same clone as 14927-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14927-1-AP (TIM22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 14927-1-AP (same clone as 14927-1-PBS)

    IP experiment of mouse liver using 14927-1-AP (same clone as 14927-1-PBS)

    IP result of anti-TIM22 (IP:14927-1-AP, 4ug; Detection:14927-1-AP 1:1000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 14927-1-AP (same clone as 14927-1-PBS)

    IF Staining of mouse brain using 14927-1-AP (same clone as 14927-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TIM22 antibody (14927-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TIM22 Polyclonal antibody, PBS Only

TIM22 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 14927-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

TIMM22, COXPD43, Testis expressed sequence 4, Testis-expressed protein 4, TEX4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14927-1-PBS targets TIM22 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6689

Product name: Recombinant human TIMM22 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-194 aa of BC002324

Sequence: MAAAAPNAGGSAPETAGSAEAPLQYSLLLQYLVGDKRQPRLLEPGSLGGIPSPAKSEEQKMIEKAMESCAFKAALACVGGFVLGGAFGVFTAGIDTNVGFDPKDPYRTPTAKEVLKDMGQRGMSYAKNFAIVGAMFSCTECLIESYRGTSDWKNSVISGCITGGAIGFRAGLKAGAIGCGGFAAFSAAIDYYLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 translocase of inner mitochondrial membrane 22 homolog (yeast)
别名 TIMM22, COXPD43, Testis expressed sequence 4, Testis-expressed protein 4, TEX4
计算分子量 20 kDa
观测分子量 20 kDa
GenBank蛋白编号BC002324
基因名称 TIM22
Gene ID (NCBI) 29928
RRIDAB_11183050
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y584
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIM22 is a translocase localized in mitochondrial inner membrane. It is implicated in import of nuclear-encoded mitochondrial preproteins to the mitochondria. TIM22 is the essential core of protein insertion complex (TIM22 complex) that consists of TIM22, TIM54, TIM18, and TIM12.

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