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验证数据展示

  • WB analysis using 26870-1-AP (same clone as 26870-1-PBS)

    WB analysis using 26870-1-AP (same clone as 26870-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26870-1-AP (TIAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

  • IF Staining of RAW 264.7 using 26870-1-AP (same clone as 26870-1-PBS)

    IF Staining of RAW 264.7 using 26870-1-AP (same clone as 26870-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TIAM2 antibody (26870-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 26870-1-AP (same clone as 26870-1-PBS)

    FC experiment of K-562 using 26870-1-AP (same clone as 26870-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human TIAM2 (26870-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

TIAM2 Polyclonal antibody, PBS Only

TIAM2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 26870-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TIAM2 Ready-To-Use IHC Kit. TIAM2 primary antibody included.

产品信息

26870-1-PBS targets TIAM2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25469

Product name: Recombinant human TIAM2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1346-1701 aa of BC137275

Sequence: KDLELTVFVFKRAVILVYKENCKLKKKLPSNSRPAHNSTDLDPFKFRWLIPISALQVRLGNPAGTENNSIWELIHTKSEIEGRPETIFQLCCSDSESKTNIVKVIRSILRENFRRHIKCELPLEKTCKDRLVPLKNRVPVSAKLASSRSLKVLKNSSSNEWTGETGKGTLLDSDEGSLSSGTQSSGCPTAEGRQDSKSTSPGKYPHPGLADFADNLIKESDILSDEDDDHRQTVKQGSPTKDIEIQFQRLRISEDPDVHPEAEQQPGPESGEGQKGGEQPKLVRGHFCPIKRKANSTKRDRGTLLKAQIRHQSLDSQSENATIDLNSVLEREFSVQSLTSVVSEECFYETESHGKS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 T-cell lymphoma invasion and metastasis 2
别名
观测分子量190-200 kDa
GenBank蛋白编号BC137275
基因名称 TIAM2
Gene ID (NCBI) 26230
RRIDAB_3085914
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IVF5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIAM2 is a Rac1-specific GEF, which was identified originally as a homologue of TIAM1. TIAM2 plays a critical role in the malignancy development of many human cancers. TIAM2 expression upregulates N-cadherin and vimentin, thus enhancing epithelial-to-mesenchymal transition (EMT) and leading to the enhancement of proliferation and invasion in hepatoma cells.

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