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TIAM2 Polyclonal antibody

TIAM2 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26870-1-AP

Print datasheet

Synonyms

KIAA2016, STEF, TIAM 2, TIAM2

验证数据展示

  • WB analysis using 26870-1-AP

    WB analysis using 26870-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26870-1-AP (TIAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of K-562 using 26870-1-AP

    FC experiment of K-562 using 26870-1-AP

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human TIAM2 (26870-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inBxPC-3 cells, U2OS cells, mouse testis tissue, rat testis tissue
Positive FC detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26870-1-AP targets TIAM2 in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen TIAM2 fusion protein Ag25469 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name T-cell lymphoma invasion and metastasis 2
Synonyms KIAA2016, STEF, TIAM 2, TIAM2
Observed Molecular Weight190-200 kDa
GenBank Accession NumberBC137275
Gene Symbol TIAM2
Gene ID (NCBI) 26230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IVF5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIAM2 is a Rac1-specific GEF, which was identified originally as a homologue of TIAM1. TIAM2 plays a critical role in the malignancy development of many human cancers. TIAM2 expression upregulates N-cadherin and vimentin, thus enhancing epithelial-to-mesenchymal transition (EMT) and leading to the enhancement of proliferation and invasion in hepatoma cells.

实验方案

Product Specific Protocols
WB protocol for TIAM2 antibody 26870-1-APDownload protocol
FC protocol for TIAM2 antibody 26870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols