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验证数据展示

  • IF Staining of HeLa using CL488-10920

    IF Staining of HeLa using CL488-10920

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 THOC1 antibody (CL488-10920) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated THOC1 Polyclonal antibody

THOC1 Polyclonal Antibody for IF/ICC
Cat No. CL488-10920

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

Nuclear matrix protein p84, HPR1, p84N5, Tho 1, THO complex 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy THOC1 Ready-To-Use IHC Kit. THOC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10920-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10920 targets THOC1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 THOC1 fusion protein Ag1352 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 THO complex 1
别名 Nuclear matrix protein p84, HPR1, p84N5, Tho 1, THO complex 1
计算分子量 84 kDa
观测分子量 84 kDa
GenBank蛋白编号BC010381
基因名称 THOC1
Gene ID (NCBI) 9984
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96FV9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

THOC1 (THO complex subunit 1), also known as Tho1, P84, HPR1 or P84N5, is a 657 amino acid nuclear matrix protein and is evolutionarily conserved from yeast to humans. THOC1 contains one death domain and is a component of the heteromultimeric THO/TREX (transcription/export) complex along with THOC2, THOC3, BAT1 and ALY. The THO/TREX complex is recruited to transcribed genes and travels along with RNA polymerase II (Pol II) during elongation, coupling elongating Pol II with RNA splicing and export factors. THOC1 is expressed at high levels in breast cancer cells and at relatively low levels in normal epithelia. A reduction of THOC1 in cancer cell lines results in promoted cancer cell apoptosis and reduced cell proliferation. This suggests that cancer cells are dependent on the high levels of THOC1 expression and therefore THOC1 may be a good target for cancer therapy. This antibody recognizes the 84kd THOC1 protein.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 THOC1 antibody CL488-10920Download protocol
Standard Protocols
Click here to view our Standard Protocols