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验证数据展示

  • WB analysis using 16782-1-AP (same clone as 16782-1-PBS)

    WB analysis using 16782-1-AP (same clone as 16782-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16782-1-AP (THEM2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16782-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 16782-1-AP (same clone as 16782-1-PBS)

    IHC staining of human lung cancer using 16782-1-AP (same clone as 16782-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16782-1-AP (THEM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16782-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACOT13/THEM2 antibody included
  • IHC staining of human lung cancer using 16782-1-AP (same clone as 16782-1-PBS)

    IHC staining of human lung cancer using 16782-1-AP (same clone as 16782-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16782-1-AP (THEM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16782-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACOT13/THEM2 antibody included
  • IF Staining of HepG2 using 16782-1-AP (same clone as 16782-1-PBS)

    IF Staining of HepG2 using 16782-1-AP (same clone as 16782-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using THEM2 antibody (16782-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16782-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 16782-1-AP (same clone as 16782-1-PBS)

    IP experiment of HeLa using 16782-1-AP (same clone as 16782-1-PBS)

    IP result of anti-THEM2 (IP:16782-1-AP, 4ug; Detection:16782-1-AP 1:2000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 16782-1-PBS in a different storage buffer formulation.

THEM2 Polyclonal antibody, PBS Only

THEM2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16782-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ACOT13/THEM2 Ready-To-Use IHC Kit. ACOT13/THEM2 primary antibody included.

产品信息

16782-1-PBS targets THEM2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10212

Product name: Recombinant human THEM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-140 aa of BC000894

Sequence: MTSMTQSLREVIKAMTKARNFERVLGKITLVSAAPGKVICEMKVEEEHTNAIGTLHGGLTATLVDNISTMALLCTERGAPGVSVDMNITYMSPAKLGEDIVITAHVLKQGKTLAFTSVDLTNKATGKLIAQGRHTKHLGN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thioesterase superfamily member 2
别名
计算分子量 140 aa, 15 kDa
观测分子量 16 kDa
GenBank蛋白编号BC000894
基因名称 THEM2
Gene ID (NCBI) 55856
RRIDAB_2287241
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NPJ3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Them2 (also named Acot13) is a homotetrameric fatty acyl-CoA thioesterase. Them2 is enriched in liver and oxidative tissues, including BAT, and is primarily associated with mitochondria.

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