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验证数据展示

  • WB analysis using 16782-1-AP

    WB analysis using 16782-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16782-1-AP (THEM2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 16782-1-AP

    IHC staining of human lung cancer using 16782-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16782-1-AP (THEM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACOT13/THEM2 antibody included
  • IHC staining of human lung cancer using 16782-1-AP

    IHC staining of human lung cancer using 16782-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16782-1-AP (THEM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACOT13/THEM2 antibody included
  • IF Staining of HepG2 using 16782-1-AP

    IF Staining of HepG2 using 16782-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using THEM2 antibody (16782-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • IP experiment of HeLa using 16782-1-AP

    IP experiment of HeLa using 16782-1-AP

    IP result of anti-THEM2 (IP:16782-1-AP, 4ug; Detection:16782-1-AP 1:2000) with HeLa cells lysate 1085 ug.

THEM2 Polyclonal antibody

THEM2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16782-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

EC:3.1.2.2, EC:3.1.2.-, Acyl-coenzyme A thioesterase 13, N-terminally processed, Acyl-coenzyme A thioesterase 13, Acyl-CoA thioesterase 13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse kidney tissue, mouse liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ACOT13/THEM2 Ready-To-Use IHC Kit. ACOT13/THEM2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16782-1-AP targets THEM2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 THEM2 fusion protein Ag10212 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thioesterase superfamily member 2
别名 EC:3.1.2.2, EC:3.1.2.-, Acyl-coenzyme A thioesterase 13, N-terminally processed, Acyl-coenzyme A thioesterase 13, Acyl-CoA thioesterase 13
计算分子量 140 aa, 15 kDa
观测分子量 16 kDa
GenBank蛋白编号BC000894
基因名称 THEM2
Gene ID (NCBI) 55856
RRIDAB_2287241
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NPJ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Them2 (also named Acot13) is a homotetrameric fatty acyl-CoA thioesterase. Them2 is enriched in liver and oxidative tissues, including BAT, and is primarily associated with mitochondria.

实验方案

Product Specific Protocols
WB protocol for THEM2 antibody 16782-1-APDownload protocol
IHC protocol for THEM2 antibody 16782-1-APDownload protocol
IF protocol for THEM2 antibody 16782-1-APDownload protocol
IP protocol for THEM2 antibody 16782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols