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THEM2 Polyclonal antibody

THEM2 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 16782-1-AP

Print datasheet

Synonyms

ACOT13, Acyl CoA thioesterase 13, HT012, PNAS 27, THEM2

验证数据展示

  • WB analysis of HeLa using 16782-1-AP

    WB analysis of HeLa using 16782-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16782-1-AP (THEM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16782-1-AP

    IHC staining of human kidney using 16782-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16782-1-AP (THEM2 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ACOT13/THEM2 antibody included
  • IHC staining of human kidney using 16782-1-AP

    IHC staining of human kidney using 16782-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16782-1-AP (THEM2 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with ACOT13/THEM2 antibody included
  • IF Staining of HepG2 using 16782-1-AP

    IF Staining of HepG2 using 16782-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16782-1-AP (THEM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, human brain tissue, human colon tissue, human liver tissue, mouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ACOT13/THEM2 Ready-To-Use IHC Kit. ACOT13/THEM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16782-1-AP targets THEM2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen THEM2 fusion protein Ag10212 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thioesterase superfamily member 2
Synonyms ACOT13, Acyl CoA thioesterase 13, HT012, PNAS 27, THEM2
Calculated Molecular Weight 140 aa, 15 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC000894
Gene Symbol THEM2
Gene ID (NCBI) 55856
RRIDAB_2287241
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPJ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for THEM2 antibody 16782-1-APDownload protocol
IHC protocol for THEM2 antibody 16782-1-APDownload protocol
IF protocol for THEM2 antibody 16782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols