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验证数据展示

  • WB analysis using 84373-6-RR

    WB analysis using 84373-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84373-6-RR (THAP11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 84373-6-RR

    WB analysis of HepG2 using 84373-6-RR

    WB result of THAP11 antibody (84373-6-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THAP11 transfected HepG2 cells.

  • IF Staining of HeLa using 84373-6-RR

    IF Staining of HeLa using 84373-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THAP11 antibody (84373-6-RR, Clone: 241590E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 84373-6-RR

    FC experiment of HepG2 using 84373-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug THAP11 Recombinant antibody (84373-6-RR, Clone:241590E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84373-6-RR

    Affinity and Kinetic Characterization of 84373-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84373-6-RR against Human THAP11 were performed. The affinity constant is below 1 pM.

  • Featured Product
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THAP11 Recombinant antibody

THAP11 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84373-6-RR

产品说明书

CloneNo. 241590E5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

241590E5, CTG B43a, CTG B45d, HRIHFB2206, RONIN

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells, HeLa cells, NCCIT cells, HT-29 cells, C2C12 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy THAP11 Ready-To-Use IHC Kit. THAP11 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84373-6-RR targets THAP11 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 THAP11 fusion protein Ag19342 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 THAP domain containing 11
别名 241590E5, CTG B43a, CTG B45d, HRIHFB2206, RONIN
计算分子量 314 aa, 34 kDa
观测分子量45 kDa
GenBank蛋白编号BC012182
基因名称 THAP11
Gene ID (NCBI) 57215
RRIDAB_3671909
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96EK4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for THAP11 antibody 84373-6-RRDownload protocol
IF protocol for THAP11 antibody 84373-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols