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  • Featured Product
  • KD/KO Validated

THADA Polyclonal antibody

THADA Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 12909-1-AP

Print datasheet

Synonyms

GITA, KIAA1767, THADA, thyroid adenoma associated

验证数据展示

  • WB analysis of CHO cells using 12909-1-AP

    WB analysis of CHO cells using 12909-1-AP

    WB result of 12909-1-AP from Takashi Kobayashi.

  • WB analysis of A549 using 12909-1-AP

    WB analysis of A549 using 12909-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 12909-1-AP

    IHC staining of human pancreas using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 12909-1-AP

    IHC staining of human pancreas using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inA549 cells, CHO cells
Positive IHC detected inhuman pancreas tissue, human testis tissue, mouse ovary tissue, rat ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12909-1-AP targets THADA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human, mouse, rat, hamster
Cited Reactivityhuman, mouse
Immunogen THADA fusion protein Ag3881 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thyroid adenoma associated
Synonyms GITA, KIAA1767, THADA, thyroid adenoma associated
Calculated Molecular Weight 220 kDa
Observed Molecular Weight 183 kDa
GenBank Accession NumberBC025773
Gene Symbol THADA
Gene ID (NCBI) 63892
RRIDAB_10638448
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6YHU6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for THADA antibody 12909-1-APDownload protocol
IHC protocol for THADA antibody 12909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF,IP

J Immunother Cancer

THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.

Authors - Chushu Li
  • KD Validated
View Article