• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HeLa using 68006-1-Ig (same clone as 68006-1-PBS)

    WB analysis of HeLa using 68006-1-Ig (same clone as 68006-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68006-1-Ig (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 68006-1-Ig (same clone as 68006-1-PBS)

    IHC staining of human liver cancer using 68006-1-Ig (same clone as 68006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68006-1-Ig (TGM2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TGM2 antibody included
  • IF Staining of A549 using 68006-1-Ig (same clone as 68006-1-PBS)

    IF Staining of A549 using 68006-1-Ig (same clone as 68006-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TGM2 antibody (68006-1-Ig, Clone: 2D4C11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68006-1-Ig (same clone as 68006-1-PBS)

    FC experiment of HeLa using 68006-1-Ig (same clone as 68006-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TGM2 (68006-1-Ig, Clone:2D4C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51456-1

    Cytometric bead array sample test of MP51456-1

    Cytometric bead array sample test of MP51456-1, TGM2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68006-1-PBS. Detection antibody: 68006-2-PBS.

  • Cytometric bead array standard curve of MP51456-1

    Cytometric bead array standard curve of MP51456-1

    Cytometric bead array standard curve of MP51456-1, TGM2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68006-1-PBS. Detection antibody: 68006-2-PBS. Standard:Ag7462. Range: 3.125-400 ng/mL.

TGM2 Monoclonal antibody, PBS Only (Capture)

TGM2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 68006-1-PBS

产品说明书

CloneNo. 2D4C11

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

2D4C11, Erythrocyte transglutaminase, TG(C), TG2, TGase 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy TGM2 Ready-To-Use IHC Kit. TGM2 primary antibody included.

产品信息

68006-1-PBS targets TGM2 as part of a matched antibody pair:

MP51456-1: 68006-1-PBS capture and 68006-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7462

Product name: Recombinant human TGM2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-349 aa of BC003551

Sequence: MAEELVLERCDLELETNGRDHHTADLCREKLVVRRGQPFWLTLHFEGRNYEASVDSLTFSVVTGPAPSQEAGTKARFPLRDAVEEGDWTATVVDQQDCTLSLQLTTPANAPIGLYRLSLEASTGYQGSSFVLGHFILLFNAWCPADAVYLDSEEERQEYVLTQQGFIYQGSAKFIKNIPWNFGQFEDGILDICLILLDVNPKFLKNAGRDCSRRSSPVYVGRVVSGMVNCNDDQGVLLGRWDNNYGDGVSPMSWIGSVDILRRWKNHGCQRVKYGQCWVFAAVACTVLRCLGIPTRVVTNYNSAHDQNSNLLIEYFRNEFGEIQGDKSEMIWNFHCWVESWMTRPDLQP

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
别名 2D4C11, Erythrocyte transglutaminase, TG(C), TG2, TGase 2
计算分子量 77 kDa
观测分子量80 kDa
GenBank蛋白编号BC003551
基因名称 TGM2
Gene ID (NCBI) 7052
ENSEMBL Gene IDENSG00000198959
RRIDAB_2918753
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP21980
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transglutaminase 2 (TGM2) is a ubiquitous and multifunctional calcium-dependent enzyme belonging to the transglutaminase family. It is best known for its canonical activity of catalyzing the cross-linking of proteins by forming stable ε-(γ-glutamyl)lysine isopeptide bonds, which contributes to extracellular matrix stabilization and wound healing. Beyond this, TGM2 exhibits GTPase activity, allowing it to function as a signaling G-protein in intracellular processes. It is implicated in a wide range of physiological functions, including cell adhesion, proliferation, and apoptosis, as well as pathological conditions such as celiac disease, fibrosis, neurodegenerative disorders, and cancer metastasis, where its dysregulated expression often contributes to disease progression.