• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HeLa using 85859-5-RR

    WB analysis of HeLa using 85859-5-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 85859-5-RR (TGFB1I1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of SKOV-3 using 85859-5-RR

    WB analysis of SKOV-3 using 85859-5-RR

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 85859-5-RR (TGFB1I1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat colon using 85859-5-RR

    WB analysis of rat colon using 85859-5-RR

    rat colon tissue were subjected to SDS PAGE followed by western blot with 85859-5-RR (TGFB1I1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

TGFB1I1 Recombinant antibody

TGFB1I1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85859-5-RR

产品说明书

CloneNo. 250139E8

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, ELISA

别名

HIC5, Androgen receptor-associated protein of 55 kDa, ARA55, Hic-5, Hydrogen peroxide-inducible clone 5 protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, rat colon tissue, SKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy TGFB1I1/HIC5 Ready-To-Use IHC Kit. TGFB1I1/HIC5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85859-5-RR targets TGFB1I1 in WB, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, rat
免疫原 TGFB1I1 fusion protein Ag0868 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transforming growth factor beta 1 induced transcript 1
别名 HIC5, Androgen receptor-associated protein of 55 kDa, ARA55, Hic-5, Hydrogen peroxide-inducible clone 5 protein
计算分子量 48 kDa
观测分子量48-50 kDa
GenBank蛋白编号BC001830
基因名称 HIC5
Gene ID (NCBI) 7041
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43294
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIC5 (Hydrogen Peroxide-Inducible Clone 5), also known as TGFB1I1, ARA55, or TSC-5. It was first identified in 1994 as a gene induced by transforming growth factor-β (TGF-β) or hydrogen peroxide (PMID: 36222304). HIC5 is a member of the Paxillin protein family and functions as a molecular adapter, delivering signals when the cellular adhesion environment changes. Given its role in cancer and fibrotic diseases, HIC5 is a potential therapeutic target. For example, targeting HIC5 or the GR tau2 region could modulate the set of genes regulated by GR, providing opportunities for intervention (PMID: 24591583).

实验方案

Product Specific Protocols
WB protocol for TGFB1I1 antibody 85859-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols