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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis of HeLa using 85859-5-RR (same clone as 85859-5-PBS)

    WB analysis of HeLa using 85859-5-RR (same clone as 85859-5-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 85859-5-RR (HIC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85859-5-PBS in a different storage buffer formulation.

  • WB analysis of SKOV-3 using 85859-5-RR (same clone as 85859-5-PBS)

    WB analysis of SKOV-3 using 85859-5-RR (same clone as 85859-5-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 85859-5-RR (HIC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85859-5-PBS in a different storage buffer formulation.

  • WB analysis of rat colon using 85859-5-RR (same clone as 85859-5-PBS)

    WB analysis of rat colon using 85859-5-RR (same clone as 85859-5-PBS)

    rat colon tissue were subjected to SDS PAGE followed by western blot with 85859-5-RR (HIC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85859-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85859-5-RR

    Affinity and Kinetic Characterization of 85859-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85859-5-RR against Human HIC5 were performed. The affinity constant is 6.15 nM.

HIC5 Recombinant monoclonal antibody, PBS Only

HIC5 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85859-5-PBS

产品说明书

CloneNo. 250139E8

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, Indirect ELISA

别名

Androgen receptor-associated protein of 55 kDa, ARA55, Hic-5, Hydrogen peroxide-inducible clone 5 protein, TGFB1I1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TGFB1I1/HIC5 Ready-To-Use IHC Kit. TGFB1I1/HIC5 primary antibody included.

产品信息

85859-5-PBS targets HIC5 in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag0868

Product name: Recombinant human TGFB1I1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-230 aa of BC001830

Sequence: MPRSGAPKERPAEPLTPPPSYGHQPQTGSGESSGASGDKDHLYSTVCKPRSPKPAAPAAPPFSSSSGVLGTGLCELDRLLQELNATQFNITDEIMSQFPSSKVASGEQKEDQSEDKKRPSLPSSPSPGLPKASATSATLELDRLMASLSDFRVQNHLPASGPTQPPVVSSTNEGSPSPPEPTGKGSLDTMLGLLQSDLSRRGVPTQAKGLCGSCNKPIAGQVVTALGRAW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transforming growth factor beta 1 induced transcript 1
别名 Androgen receptor-associated protein of 55 kDa, ARA55, Hic-5, Hydrogen peroxide-inducible clone 5 protein, TGFB1I1
计算分子量 48 kDa
观测分子量48-50 kDa
GenBank蛋白编号BC001830
基因名称 HIC5
Gene ID (NCBI) 7041
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43294
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIC5 (Hydrogen Peroxide-Inducible Clone 5), also known as TGFB1I1, ARA55, or TSC-5. It was first identified in 1994 as a gene induced by transforming growth factor-β (TGF-β) or hydrogen peroxide (PMID: 36222304). HIC5 is a member of the Paxillin protein family and functions as a molecular adapter, delivering signals when the cellular adhesion environment changes. Given its role in cancer and fibrotic diseases, HIC5 is a potential therapeutic target. For example, targeting HIC5 or the GR tau2 region could modulate the set of genes regulated by GR, providing opportunities for intervention (PMID: 24591583).