• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TFPI Polyclonal antibody

TFPI Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20865-1-AP

Print datasheet

Synonyms

EPI, Extrinsic pathway inhibitor, LACI, TFI, TFPI, TFPI1

验证数据展示

  • WB analysis of HeLa using 20865-1-AP

    WB analysis of HeLa using 20865-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20865-1-AP (TFPI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 20865-1-AP

    IF Staining of HepG2 using 20865-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20865-1-AP (TFPI antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20865-1-AP is TFPI Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen TFPI fusion protein Ag14913 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
Synonyms EPI, Extrinsic pathway inhibitor, LACI, TFI, TFPI, TFPI1
Calculated Molecular Weight251aa,29 kDa; 304aa,35 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC015514
Gene Symbol TFPI
Gene ID (NCBI) 7035
RRIDAB_10693682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10646
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.

实验方案

Product Specific Protocols
WB protocol for TFPI antibody 20865-1-APDownload protocol
IF protocol for TFPI antibody 20865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Gene

ADTRP regulates TFPI expression via transcription factor POU1F1 involved in coronary artery disease.

Authors - Chunyan Luo
View Article
humanIHC

Clin Appl Thromb Hemost

Endocardial Changes in Nonvalvular Atrial Fibrillation Without Atrial Thrombus-Thrombomodulin and Tissue Factor Pathway Inhibitor.

Authors - Kang An
View Article
humanWB

Thromb Res

Activated protein C, protein S, and tissue factor pathway inhibitor cooperate to inhibit thrombin activation

Authors - Xian Li
View Article