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验证数据展示

  • WB analysis using 13734-1-AP (same clone as 13734-1-PBS)

    WB analysis using 13734-1-AP (same clone as 13734-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13734-1-AP (TFF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 13734-1-AP (same clone as 13734-1-PBS)

    IHC staining of human stomach using 13734-1-AP (same clone as 13734-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TFF1 antibody included
  • IHC staining of human stomach using 13734-1-AP (same clone as 13734-1-PBS)

    IHC staining of human stomach using 13734-1-AP (same clone as 13734-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TFF1 antibody included
  • IHC staining of human colon cancer using 13734-1-AP (same clone as 13734-1-PBS)

    IHC staining of human colon cancer using 13734-1-AP (same clone as 13734-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TFF1 antibody included
  • IHC staining of human ovary tumor using 13734-1-AP (same clone as 13734-1-PBS)

    IHC staining of human ovary tumor using 13734-1-AP (same clone as 13734-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TFF1 antibody included
  • IHC staining of human stomach cancer using 13734-1-AP (same clone as 13734-1-PBS)

    IHC staining of human stomach cancer using 13734-1-AP (same clone as 13734-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TFF1 antibody included
  • IF Staining of MCF-7 using 13734-1-AP (same clone as 13734-1-PBS)

    IF Staining of MCF-7 using 13734-1-AP (same clone as 13734-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Trefoil factor 1 antibody (13734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 13734-1-AP (same clone as 13734-1-PBS)

    IHC staining of human stomach cancer using 13734-1-AP (same clone as 13734-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13734-1-AP (TFF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TFF1 antibody included
  • Featured Product
  • KD/KO Validated

TFF1 Polyclonal antibody, PBS Only

TFF1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13734-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Breast cancer estrogen-inducible protein, HP1.A, pNR 2, PNR-2, Polypeptide P1.A

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TFF1 Ready-To-Use IHC Kit. TFF1 primary antibody included.

产品信息

13734-1-PBS targets TFF1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4541

Product name: Recombinant human TFF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-84 aa of BC032811

Sequence: MATMENKVICALVLVSMLALGTLAEAQTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFYPNTIDVPPEEECEF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trefoil factor 1
别名 Breast cancer estrogen-inducible protein, HP1.A, pNR 2, PNR-2, Polypeptide P1.A
计算分子量 84 aa, 9 kDa
观测分子量 9-12 kDa
GenBank蛋白编号BC032811
基因名称 TFF1
Gene ID (NCBI) 7031
RRIDAB_2202853
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04155
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.

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