验证数据展示

WB analysis using 13734-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13734-1-AP (TFF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TFF1 antibody included
IHC staining of human stomach using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TFF1 antibody included
IHC staining of human colon cancer using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TFF1 antibody included
IHC staining of human ovary tumor using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TFF1 antibody included
IHC staining of human stomach cancer using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TFF1 antibody included
IF Staining of MCF-7 using 13734-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Trefoil factor 1 antibody (13734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC staining of human stomach cancer using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13734-1-AP (TFF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TFF1 antibody included

Featured Product
KD/KO验证

TFF1 Polyclonal antibody

TFF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13734-1-AP

产品说明书

引用文献(12)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Breast cancer estrogen-inducible protein, HP1.A, pNR 2, PNR-2, Polypeptide P1.A

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (12)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	MCF-7 cells, human kidney tissue, mouse stomach tisssue
Positive IHC detected in	human stomach cancer tissue, human colon cancer tissue, human ovary tumor tissue, human stomach tissue, mouse stomach tissue, rat stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Planning an IHC experiment? We recommend our IHCeasy TFF1 Ready-To-Use IHC Kit. TFF1 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 3 publications below
WB	See 8 publications below
IHC	See 6 publications below
IF	See 1 publications below

产品信息

13734-1-AP targets TFF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, rat
免疫原	CatNo: Ag4541 Product name: Recombinant human TFF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-84 aa of BC032811 Sequence: MATMENKVICALVLVSMLALGTLAEAQTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFYPNTIDVPPEEECEF 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	trefoil factor 1
别名	Breast cancer estrogen-inducible protein, HP1.A, pNR 2, PNR-2, Polypeptide P1.A
计算分子量	84 aa, 9 kDa
观测分子量	9-12 kDa
GenBank蛋白编号	BC032811
基因名称	TFF1
Gene ID (NCBI)	7031
RRID	AB_2202853
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P04155
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.

实验方案

Product Specific Protocols
WB protocol for TFF1 antibody 13734-1-AP	Download protocol
IHC protocol for TFF1 antibody 13734-1-AP	Download protocol
IF protocol for TFF1 antibody 13734-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IF	J Exp Med Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. Authors - Yalong Wang View Article
human	IHC	JCI Insight Resolving the difference between left-sided and right-sided colorectal cancer by single-cell sequencing. Authors - Wei Guo View Article
human	WB,IHC	Bone Trefoil factor-1 upregulation in estrogen-receptor positive breast cancer correlates with an increased risk of bone metastasis. Authors - Chiara Spadazzi KO Validated View Article
human	WB,IHC	Biomed Pharmacother Trefoil factor 1 (TFF1) is a potential prognostic biomarker with functional significance in breast cancers. Authors - Jie Yi KD Validated View Article
human	WB	Aesthet Surg J Cell-Free Fat Extract Prevents Vaginal Atrophy in Ovariectomized Model Via Promoting Proliferation of Vaginal Keratinocyte and Neovascularization. Authors - Bijun Kang View Article
human	IHC	BMC Med Genomics Distinctions in gastric cancer gene expression signatures derived from laser capture microdissection versus histologic macrodissection. Authors - Kim Hark Kyun HK View Article

验证数据展示

WB analysis using 13734-1-AP

IHC staining of human stomach using 13734-1-AP

IHC staining of human stomach using 13734-1-AP

IHC staining of human colon cancer using 13734-1-AP

IHC staining of human ovary tumor using 13734-1-AP

IHC staining of human stomach cancer using 13734-1-AP

IF Staining of MCF-7 using 13734-1-AP

IHC staining of human stomach cancer using 13734-1-AP

TFF1 Polyclonal antibody

TFF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.

Resolving the difference between left-sided and right-sided colorectal cancer by single-cell sequencing.

Trefoil factor-1 upregulation in estrogen-receptor positive breast cancer correlates with an increased risk of bone metastasis.

Trefoil factor 1 (TFF1) is a potential prognostic biomarker with functional significance in breast cancers.

Cell-Free Fat Extract Prevents Vaginal Atrophy in Ovariectomized Model Via Promoting Proliferation of Vaginal Keratinocyte and Neovascularization.

Distinctions in gastric cancer gene expression signatures derived from laser capture microdissection versus histologic macrodissection.