验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84408-4 targets TFDP2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag2054 Product name: Recombinant human TFDP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 38-387 aa of BC021113 Sequence: GWVPGDRKRARKFIDSDFSESKRSKKGDKNGKGLRHFSMKVCEKVQRKGTTSYNEVADELVSEFTNSNNHLAADSQAYDQKNIRRRVYDALNVLMAMNIISKEKKEIKWIGLPTNSAQECQNLEIEKQRRIERIKQKRAQLQELLLQQIAFKNLVQRNRQNEQQNQGPPALNSTIQLPFIIINTSRKTVIDCSISSDKFEYLFNFDNTFEIHDDIEVLKRMGMSFGLESGKCSLEDLKLAKSLVPKALEGYITDISTGPSWLNQGLLLNSTQSVSNLDLTTGATLPQSSVNQGLCLDAEVALATGQFLAPNSHQSSSAASHCSESRGETPCSFNDEDEEDDEEDSSSPE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | transcription factor Dp-2 (E2F dimerization partner 2) |
| 别名 | Transcription factor Dp-2, DP2, Dp 2, 241457A12 |
| 计算分子量 | 446 aa, 49 kDa |
| GenBank蛋白编号 | BC021113 |
| 基因名称 | TFDP2 |
| Gene ID (NCBI) | 7029 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q14188 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TFDP2, also known as E2FDP2, is a coactivator of E2F factors and involves in EGF or IFN-gamma-mediated effects by enhancing or reducing active heterodimeric complexes. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1/DP complex appears to mediate both cell proliferation and apoptosis [PMID:16360038]. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of several genes whose products are involved in cell cycle regulation or DNA replication [PMID:16721044].
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 TFDP2 antibody CL488-84408-4 | Download protocol |
| IF protocol for CL Plus 488 TFDP2 antibody CL488-84408-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
