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验证数据展示

  • WB analysis using 82745-1-RR (same clone as 82745-1-PBS)

    WB analysis using 82745-1-RR (same clone as 82745-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82745-1-RR (TFAM antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82745-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82745-1-RR (same clone as 82745-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82745-1-RR (same clone as 82745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82745-1-RR (TFAM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82745-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82745-1-RR (same clone as 82745-1-PBS)

    IF Staining of HepG2 using 82745-1-RR (same clone as 82745-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (82745-1-RR, Clone: 4L4 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82745-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82745-1-RR

    Affinity and Kinetic Characterization of 82745-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82745-1-RR against Human TFAM were performed. The affinity constant is 45.7 pM.

TFAM Recombinant antibody, PBS Only

TFAM Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82745-1-PBS

产品说明书

CloneNo. 4L4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Transcription factor 6-like 2, TCF-6, TCF6, mtTFA, MtTF1

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82745-1-PBS targets TFAM in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 TFAM fusion protein Ag18299 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transcription factor A, mitochondrial
别名 Transcription factor 6-like 2, TCF-6, TCF6, mtTFA, MtTF1
计算分子量 246 aa, 29 kDa
观测分子量24-25 kDa
GenBank蛋白编号BC126366
基因名称 TFAM
Gene ID (NCBI) 7019
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ00059
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.