验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, SKOV-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27150-1-AP targets TET3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag25877 Product name: Recombinant human TET3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 316-360 aa of BC022243 Sequence: KKIQKEKLSTPEKIKQEALELAGITSDPGLSLKGGLSQQGLKPSL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tet oncogene family member 3 |
| 别名 | tet oncogene family member 3, Methylcytosine dioxygenase TET3, KIAA0401, EC:1.14.11.80 |
| 计算分子量 | 1660 aa, 179 kDa |
| 观测分子量 | 180 kDa |
| GenBank蛋白编号 | BC022243 |
| 基因名称 | TET3 |
| Gene ID (NCBI) | 200424 |
| RRID | AB_3669585 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43151 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ten-eleven translocation protein 3 (TET3) belongs to the family of ten-eleven translocations (TETs) which induce DNA demethylation and gene regulation at the epigenetic level by converting 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) (PMID: 31656201). In ovarian cancer, TET3 is reported as an oncogene or tumor suppressor during tumorigenesis and overexpressed in ovarian cancer tissues (PMID: 27141829).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TET3 antibody 27150-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
