验证数据展示
经过测试的应用
| Positive FC detected in | mouse bone marrow cells |
For other applications, we recommend the unconjugated version of this antibody, 65149-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65149 targets TER-119 in FC applications and shows reactivity with Mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | Mouse |
| 免疫原 |
Day-14 fetal liver cells from a C57BL/6 mouse 种属同源性预测 |
| 宿主/亚型 | Rat / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | lymphocyte antigen 76 |
| 别名 | Ly76, lymphocyte antigen 76, TER 119, Ter119, TER-119 |
| 基因名称 | TER-119 |
| Gene ID (NCBI) | 104231 |
| RRID | AB_2883206 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
TER-119 is a mouse erythroid lineage-specific monoclonal antibody that reacts with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte, but not with cells showing typical erythroid blast-forming unit (BFU-E) and erythroid colony-forming unit (CFU-E) activities (PMID: 1975515; 10848813). TER-119 recognizes a 52-kDa molecule on erythrocyte membranes (PMID: 10848813). TER-119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself (PMID: 10848813).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 TER-119 antibody CL488-65149 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
