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TELO2 Monoclonal antibody

TELO2 Monoclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3H10F4

Cat No : 66077-1-Ig

Print datasheet

Synonyms

hCLK2, KIAA0683, Protein clk 2 homolog, TEL2, TELO2

验证数据展示

  • WB analysis using 66077-1-Ig

    WB analysis using 66077-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of Y79 using 66077-1-Ig

    WB analysis of Y79 using 66077-1-Ig

    Y79 cells were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 66077-1-Ig

    IP experiment of HepG2 using 66077-1-Ig

    IP result of anti-TELO2 (IP:66077-1-Ig, 4ug; Detection:66077-1-Ig 1:500) with HepG2 cells lysate 2900ug.

  • IF Staining of HepG2 using 66077-1-Ig

    IF Staining of HepG2 using 66077-1-Ig

    Immunofluorescent analysis of () fixed HepG2 cells using 66077-1-Ig (TELO2 antibody) at dilution of 1:25.

  • IHC staining of human kidney using 66077-1-Ig

    IHC staining of human kidney using 66077-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66077-1-Ig (TELO2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 66077-1-Ig

    IHC staining of human kidney using 66077-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66077-1-Ig (TELO2 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inY79 cells, L02 cells, A431 cells, HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66077-1-Ig targets TELO2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, rat
Cited Reactivityhuman
Immunogen TELO2 fusion protein Ag8834 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TEL2, telomere maintenance 2, homolog (S. cerevisiae)
Synonyms hCLK2, KIAA0683, Protein clk 2 homolog, TEL2, TELO2
Calculated Molecular Weight 837 aa, 92 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC017188
Gene Symbol TELO2
Gene ID (NCBI) 9894
RRIDAB_11182835
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y4R8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TELO2 gene encodes a telomere length regulation protein TEL2 homolog. TELO2 may be involved in telomere length regulation and can form TTT complex with TTI1 and TTI2. TTT complex is required to stabilize protein levels of PIKK family proteins and is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), and ultraviolet (UV). The activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals, can be promoted, stabilized and maintained by TELO2.

实验方案

Product Specific Protocols
WB protocol for TELO2 antibody 66077-1-IgDownload protocol
IHC protocol for TELO2 antibody 66077-1-IgDownload protocol
IF protocol for TELO2 antibody 66077-1-IgDownload protocol
IP protocol for TELO2 antibody 66077-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Inositol Pyrophosphates Mediate the DNA-PK/ATM-p53 Cell Death Pathway by Regulating CK2 Phosphorylation of Tti1/Tel2.

Authors - Feng Rao
View Article