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验证数据展示

  • IF Staining of MCF-7 using 13120-1-AP (same clone as 13120-1-PBS)

    IF Staining of MCF-7 using 13120-1-AP (same clone as 13120-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13120-1-AP (TEAD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

  • WB analysis using 13120-1-AP (same clone as 13120-1-PBS)

    WB analysis using 13120-1-AP (same clone as 13120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13120-1-AP (TEAD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

  • IHC staining of rat heart using 13120-1-AP (same clone as 13120-1-PBS)

    IHC staining of rat heart using 13120-1-AP (same clone as 13120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13120-1-AP (TEAD3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

  • WB analysis using 13120-1-AP (same clone as 13120-1-PBS)

    WB analysis using 13120-1-AP (same clone as 13120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13120-1-AP (TEAD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse intestine using 13120-1-AP (same clone as 13120-1-PBS)

    IHC staining of mouse intestine using 13120-1-AP (same clone as 13120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 13120-1-AP (TEAD3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

TEAD3 Polyclonal antibody, PBS Only

TEAD3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13120-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DTEF 1, DTEF-1, ETFR 1, TEA domain family member 3, TEAD 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13120-1-PBS targets TEAD3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3875

Product name: Recombinant human TEAD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-324 aa of BC027877

Sequence: MNLDQVSKDKALQSMASMSSAQIVSASVLQNKFSPPSPLPQAVFSTSSRFWSSPPLLGQQPGPSQDIKPFAQPAYPIQPPLPPTLSSYEPLAPLPSAAASVPVWQDRTIASSRLRLLEYSAFMEVQRDPDTYSKHLFVHIGQTNPAFSDPPLEAVDVRQIYDKFPEKKGGLKELYEKGPPNAFFLVKFWADLNSTIQEGPGAFYGVSSQYSSADSMTISVSTKVCSFGKQVVEKVETEYARLENGRFVYRIHRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTSRDSQETLLVIAFVFEVSTSEHGAQHHVYKLVKD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TEA domain family member 3
别名 DTEF 1, DTEF-1, ETFR 1, TEA domain family member 3, TEAD 3
计算分子量 435 aa, 49 kDa
观测分子量50 kDa
GenBank蛋白编号BC027877
基因名称 TEAD3
Gene ID (NCBI) 7005
RRIDAB_2203068
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99594
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TEAD3, others named ranscriptional enhancer factor 5(TEF5), a member of family of transcription factors that plays a key role in the Hippo signaling pathway, suggesting involve in organ and tumor suppression by restricting proliferation and promoting. TEAD3 contains the TEA/ATTS DNA-binding domain. Human TEAD3 has a strong mRNA expression in placenta and choriocarcinoma cells(JEG-3), but low in Hela, HepG2 and MCF-7. This is a rabbit polyclonal anti-TEAD3 antibody raised against part of C-terminal chain of human TEAD3.

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