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TEAD3 Polyclonal antibody

TEAD3 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13120-1-AP

Print datasheet

Synonyms

DTEF 1, ETFR 1, TEA domain family member 3, TEAD 3, TEAD3, TEAD5, TEF 5, TEF5

验证数据展示

  • IF Staining of MCF-7 using 13120-1-AP

    IF Staining of MCF-7 using 13120-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13120-1-AP (TEAD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 13120-1-AP

    WB analysis using 13120-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13120-1-AP (TEAD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHCT 116 cells, HepG2 cells, MCF-7 cells
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13120-1-AP targets TEAD3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen TEAD3 fusion protein Ag3875 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TEA domain family member 3
Synonyms DTEF 1, ETFR 1, TEA domain family member 3, TEAD 3, TEAD3, TEAD5, TEF 5, TEF5
Calculated Molecular Weight 435 aa, 49 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC027877
Gene Symbol TEAD3
Gene ID (NCBI) 7005
RRIDAB_2203068
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99594
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TEAD3, others named ranscriptional enhancer factor 5(TEF5), a member of family of transcription factors that plays a key role in the Hippo signaling pathway, suggesting involve in organ and tumor suppression by restricting proliferation and promoting. TEAD3 contains the TEA/ATTS DNA-binding domain. Human TEAD3 has a strong mRNA expression in placenta and choriocarcinoma cells(JEG-3), but low in Hela, HepG2 and MCF-7. This is a rabbit polyclonal anti-TEAD3 antibody raised against part of C-terminal chain of human TEAD3.

实验方案

Product Specific Protocols
WB protocol for TEAD3 antibody 13120-1-APDownload protocol
IF protocol for TEAD3 antibody 13120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Cell Biol

Thyroid hormone regulates muscle fiber type conversion via miR-133a1.

Authors - Duo Zhang
View Article
mouseWB

Res Sq

Long noncoding RNA Malat1 inhibits Tead3-Nfatc1-mediated osteoclastogenesis to suppress osteoporosis and bone metastasis

Authors - Yang Zhao
View Article
humanWB

Sci Adv

TEAD4 is a master regulator of high-risk nasopharyngeal carcinoma

Authors - Ya-Qin Wang
View Article
mouseIHC

Adv Sci (Weinh)

SUMOylation of TEAD1 Modulates the Mechanism of Pathological Cardiac Hypertrophy

Authors - Xin Shi
View Article