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验证数据展示

  • WB analysis of MCF-7 using 10641-1-AP (same clone as 10641-1-PBS)

    WB analysis of MCF-7 using 10641-1-AP (same clone as 10641-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10641-1-AP (TDP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10641-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 10641-1-AP (same clone as 10641-1-PBS)

    IP experiment of HeLa using 10641-1-AP (same clone as 10641-1-PBS)

    IP result of anti-TDP1 (IP:10641-1-AP, 4ug; Detection:10641-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 10641-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 10641-1-AP (same clone as 10641-1-PBS)

    IHC staining of human ovary cancer using 10641-1-AP (same clone as 10641-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10641-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 10641-1-AP (same clone as 10641-1-PBS)

    IHC staining of human ovary cancer using 10641-1-AP (same clone as 10641-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10641-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 10641-1-AP (same clone as 10641-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 10641-1-AP (same clone as 10641-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10641-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 10641-1-AP (same clone as 10641-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 10641-1-AP (same clone as 10641-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10641-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TDP1 Polyclonal antibody, PBS Only

TDP1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10641-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

Tyrosyl-DNA phosphodiesterase 1, Tyr-DNA phosphodiesterase 1, Tyr DNA phosphodiesterase 1, EC:3.1.4.-

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10641-1-PBS targets TDP1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0964

Product name: Recombinant human TDP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-298 aa of BC006083

Sequence: MSQEGDYGRWTISSSDESEEEKPKPDKPSTSSLLCARQGAANEPRYTCSEAQKAAHKRKISPVKFSNTDSVLPPKRQKSGSQEDLGWCLSSSDDELQPEMPQKQAEKVVIKKEKDISAPNDGTAQRTENHGAPACHRLKEEEDEYETSGEGQDIWDMLDKGNPFQFYLTRVSGVKPKYNSGALHIKDILSPLFGTLVSSAQFNYCFDVDWLVKQYPPEFRKKPILLVHGDKREAKAHLHAQAKPYENISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIHADWHQKTQGTHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tyrosyl-DNA phosphodiesterase 1
别名 Tyrosyl-DNA phosphodiesterase 1, Tyr-DNA phosphodiesterase 1, Tyr DNA phosphodiesterase 1, EC:3.1.4.-
计算分子量 34 kDa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC006083
基因名称 TDP1
Gene ID (NCBI) 55775
RRIDAB_2240520
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NUW8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tyrosyl DNA phosphodiesterase 1 (TDP1) is an enzyme capable of hydrolyzing phosphodiester bonds between tyrosine and the 3-phosphate of DNA, which are typically generated in a transient manner by DNA topoisomerase I (topo I) and it appears to be more mobile and diffusible than topo I, and it has a different distribution in the nucleus (PMID:15494395). TDP1 is involed in protecting cells against oxidative DNA damage, and with the impaired ability of TDP1-deficient cells to remove 3-phosphoglycolate (PMID:21041670). Defects in TDP1 are the cause of spinocerebellar ataxia autosomal recessive with axonal neuropathy (SCAN1)(PMID:17948061).

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