验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy TCOF1 Ready-To-Use IHC Kit. TCOF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84914-4-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-84914-4 targets TCOF1 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | TCOF1 fusion protein Ag1447 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Treacher Collins-Franceschetti syndrome 1 |
| 别名 | 242201E12, MFD1, treacle |
| 计算分子量 | 97 kDa, 144 kDa, 152 kDa |
| 观测分子量 | 220-240 kDa |
| GenBank蛋白编号 | BC014559 |
| 基因名称 | TCOF1 |
| Gene ID (NCBI) | 6949 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13428 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TCOF1 also known as treacle, is a nucleolar protein with a LIS1 homology domain. TCOF1 is involved in ribosomal DNA gene transcription by interacting with upstream binding factor (UBF). Mutations in TCOF1 have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development(PMID: 31218872)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 TCOF1 antibody CL594-84914-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |