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验证数据展示

  • FC experiment of A431 using CL488-67652

    FC experiment of A431 using CL488-67652

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TCF7L1/TCF7L2 (CL488-67652, Clone:2B2D12) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated TCF7L1/TCF7L2 Monoclonal antibody

TCF7L1/TCF7L2 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67652

产品说明书

CloneNo. 2B2D12

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse

应用

FC (Intra)

别名

TCF7L1, TCF7L2, TCF4, TCF-3, TCF3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67652-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67652 targets TCF7L1/TCF7L2 in FC (Intra) applications and shows reactivity with Human, Mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Mouse
免疫原 TCF7L1/TCF7L2 fusion protein Ag28954 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 transcription factor 7-like 1 (T-cell specific, HMG-box)
别名 TCF7L1, TCF7L2, TCF4, TCF-3, TCF3
计算分子量 63 kDa
观测分子量 70 kDa
GenBank蛋白编号BC058894
基因名称 TCF7L1
Gene ID (NCBI) 83439
RRIDAB_2934557
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9HCS4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis. This antibody raised against 1-200aa of human TCF7L1 can also react with TCF7L2.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TCF7L1/TCF7L2 antibody CL488-67652Download protocol
Standard Protocols
Click here to view our Standard Protocols