• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67523-1-Ig (same clone as 67523-1-PBS)

    WB analysis using 67523-1-Ig (same clone as 67523-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67523-1-Ig (TCF12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67523-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 67523-1-Ig (same clone as 67523-1-PBS)

    FC experiment of HepG2 using 67523-1-Ig (same clone as 67523-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human TCF12 (67523-1-Ig, Clone:1B8D4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67523-1-PBS in a different storage buffer formulation.

TCF12 Monoclonal antibody, PBS Only

TCF12 Monoclonal Antibody for Indirect ELISA, WB
Cat No. 67523-1-PBS

产品说明书

CloneNo. 1B8D4

宿主/亚型

Mouse / IgG2a

种属反应性

rat, mouse, human

应用

Indirect ELISA, WB

别名

TCF12/HEB, E-box-binding protein, E box binding protein, DNA-binding protein HTF4, Class B basic helix-loop-helix protein 20

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy TCF12 Ready-To-Use IHC Kit. TCF12 primary antibody included.

产品信息

67523-1-PBS targets TCF12 in Indirect ELISA, WB applications and shows reactivity with rat, mouse, human samples.

经测试应用 Indirect ELISA, WB Application Description
经测试反应性 rat, mouse, human
免疫原

CatNo: Ag6287

Product name: Recombinant human TCF12 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 358-706 aa of BC050556

Sequence: SNPSTPVGSPSPLTGTSQWPRPGGQAPSSPSYENSLHSLKNRVEQQLHEHLQDAMSFLKDVCEQSRMEDRLDRLDDAIHVLRNHAVGPSTSLPAGHSDIHSLLGPSHNAPIGSLNSNYGGSSLVASSRSASMVGTHREDSVSLNGNHSVLSSTVTTSSTDLNHKTQENYRGGLQSQSGTVVTTEIKTENKEKDENLHEPPSSDDMKSDDESSQKDIKVSSRGRTSSTNEDEDLNPEQKIEREKERRMANNARERLRVRDINEAFKELGRMCQLHLKSEKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSAVSAEPPTTLPGTHPGLSETTNPMGHM

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 transcription factor 12
别名 TCF12/HEB, E-box-binding protein, E box binding protein, DNA-binding protein HTF4, Class B basic helix-loop-helix protein 20
计算分子量 73 kDa
观测分子量 80 kDa
GenBank蛋白编号BC050556
基因名称 TCF12
Gene ID (NCBI) 6938
RRIDAB_2882743
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ99081
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The product of TCF12 gene (TCF12, also called HTF4 or HEB) is a member of helix-loop-helix (HLH) protein family [PMID:22130667]. TCF12 is a transcriptional regulator that involved in the initiation of neuronal differentiation. It 's also known as E proteins, and activates transcription by binding to the E box (5'-CANNTG-3'), because of their direct DNA (E-box) binding ability [PMID: 19204326]. TCF12 was shown to suppress E-cadherin expression during the early stages of renal tubular epithelial cell dedifferentiation [PMID:17202424].