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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67523-1-Ig (same clone as 67523-1-PBS)

    WB analysis using 67523-1-Ig (same clone as 67523-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67523-1-Ig (TCF12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67523-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 67523-1-Ig (same clone as 67523-1-PBS)

    FC experiment of HepG2 using 67523-1-Ig (same clone as 67523-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human TCF12 (67523-1-Ig, Clone:1B8D4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67523-1-PBS in a different storage buffer formulation.

TCF12 Monoclonal antibody, PBS Only

TCF12 Monoclonal Antibody for Indirect ELISA, WB
Cat No. 67523-1-PBS

产品说明书

CloneNo. 1B8D4

宿主/亚型

Mouse / IgG2a

种属反应性

rat, mouse, human

应用

Indirect ELISA, WB

别名

TCF12/HEB, E-box-binding protein, E box binding protein, DNA-binding protein HTF4, Class B basic helix-loop-helix protein 20

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TCF12 Ready-To-Use IHC Kit. TCF12 primary antibody included.

产品信息

67523-1-PBS targets TCF12 in Indirect ELISA, WB applications and shows reactivity with rat, mouse, human samples.

经测试应用 Indirect ELISA, WB Application Description
经测试反应性 rat, mouse, human
免疫原 TCF12 fusion protein Ag6287 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 transcription factor 12
别名 TCF12/HEB, E-box-binding protein, E box binding protein, DNA-binding protein HTF4, Class B basic helix-loop-helix protein 20
计算分子量 73 kDa
观测分子量 80 kDa
GenBank蛋白编号BC050556
基因名称 TCF12
Gene ID (NCBI) 6938
RRIDAB_2882743
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99081
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The product of TCF12 gene (TCF12, also called HTF4 or HEB) is a member of helix-loop-helix (HLH) protein family [PMID:22130667]. TCF12 is a transcriptional regulator that involved in the initiation of neuronal differentiation. It 's also known as E proteins, and activates transcription by binding to the E box (5'-CANNTG-3'), because of their direct DNA (E-box) binding ability [PMID: 19204326]. TCF12 was shown to suppress E-cadherin expression during the early stages of renal tubular epithelial cell dedifferentiation [PMID:17202424].