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验证数据展示

  • WB analysis using 67523-1-Ig

    WB analysis using 67523-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67523-1-Ig (TCF12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 67523-1-Ig

    FC experiment of HepG2 using 67523-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human TCF12 (67523-1-Ig, Clone:1B8D4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

TCF12 Monoclonal antibody

TCF12 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67523-1-Ig

产品说明书

CloneNo. 1B8D4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

TCF12/HEB, E-box-binding protein, E box binding protein, DNA-binding protein HTF4, Class B basic helix-loop-helix protein 20

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TCF12 Ready-To-Use IHC Kit. TCF12 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67523-1-Ig targets TCF12 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6287

Product name: Recombinant human TCF12 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 358-706 aa of BC050556

Sequence: SNPSTPVGSPSPLTGTSQWPRPGGQAPSSPSYENSLHSLKNRVEQQLHEHLQDAMSFLKDVCEQSRMEDRLDRLDDAIHVLRNHAVGPSTSLPAGHSDIHSLLGPSHNAPIGSLNSNYGGSSLVASSRSASMVGTHREDSVSLNGNHSVLSSTVTTSSTDLNHKTQENYRGGLQSQSGTVVTTEIKTENKEKDENLHEPPSSDDMKSDDESSQKDIKVSSRGRTSSTNEDEDLNPEQKIEREKERRMANNARERLRVRDINEAFKELGRMCQLHLKSEKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSAVSAEPPTTLPGTHPGLSETTNPMGHM

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 transcription factor 12
别名 TCF12/HEB, E-box-binding protein, E box binding protein, DNA-binding protein HTF4, Class B basic helix-loop-helix protein 20
计算分子量 73 kDa
观测分子量 80 kDa
GenBank蛋白编号BC050556
基因名称 TCF12
Gene ID (NCBI) 6938
RRIDAB_2882743
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ99081
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The product of TCF12 gene (TCF12, also called HTF4 or HEB) is a member of helix-loop-helix (HLH) protein family [PMID:22130667]. TCF12 is a transcriptional regulator that involved in the initiation of neuronal differentiation. It 's also known as E proteins, and activates transcription by binding to the E box (5'-CANNTG-3'), because of their direct DNA (E-box) binding ability [PMID: 19204326]. TCF12 was shown to suppress E-cadherin expression during the early stages of renal tubular epithelial cell dedifferentiation [PMID:17202424].

实验方案

Product Specific Protocols
WB protocol for TCF12 antibody 67523-1-IgDownload protocol
FC protocol for TCF12 antibody 67523-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols