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验证数据展示

  • WB analysis using 16104-1-AP

    WB analysis using 16104-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16104-1-AP (Telethonin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 16104-1-AP

    IHC staining of mouse heart using 16104-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16104-1-AP (Telethonin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Telethonin antibody included
  • IHC staining of mouse skeletal muscle using 16104-1-AP

    IHC staining of mouse skeletal muscle using 16104-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16104-1-AP (Telethonin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Telethonin antibody included

Telethonin Polyclonal antibody

Telethonin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16104-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

TCAP, CMD1N

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Telethonin Ready-To-Use IHC Kit. Telethonin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16104-1-AP targets Telethonin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9014

Product name: Recombinant human TCAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-167 aa of BC012628

Sequence: MATSELSCEVSEENCERREAFWAEWKDLTLSTRPEEGCSLHEEDTQRHETYHQQGQCQVLVQRSPWLMMRMGILGRGLQEYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALETALGGQCVDRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEAQRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 titin-cap (telethonin)
别名 TCAP, CMD1N
计算分子量 167 aa, 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC012628
基因名称 TCAP
Gene ID (NCBI) 8557
RRIDAB_3741877
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15273
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Telethonin, also known as TCAP, is a sarcomeric protein found exclusively in striated and cardiac muscle, where it localizes to the periphery of Z discs that define the border of the sarcomere. It binds to the N-terminus of myoglobin, effectively increasing its mechanical resistance. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G(PMID: 19679566).

实验方案

Product Specific Protocols
IHC protocol for Telethonin antibody 16104-1-APDownload protocol
WB protocol for Telethonin antibody 16104-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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