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验证数据展示

  • WB analysis using 16104-1-AP

    WB analysis using 16104-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16104-1-AP (Telethonin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 16104-1-AP

    IHC staining of mouse heart using 16104-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16104-1-AP (Telethonin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Telethonin antibody included
  • IHC staining of mouse skeletal muscle using 16104-1-AP

    IHC staining of mouse skeletal muscle using 16104-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16104-1-AP (Telethonin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Telethonin antibody included

Telethonin Polyclonal antibody

Telethonin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16104-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

TCAP, CMD1N

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Telethonin Ready-To-Use IHC Kit. Telethonin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16104-1-AP targets Telethonin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9014

Product name: Recombinant human TCAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-167 aa of BC012628

Sequence: MATSELSCEVSEENCERREAFWAEWKDLTLSTRPEEGCSLHEEDTQRHETYHQQGQCQVLVQRSPWLMMRMGILGRGLQEYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALETALGGQCVDRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEAQRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 titin-cap (telethonin)
别名 TCAP, CMD1N
计算分子量 167 aa, 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC012628
基因名称 TCAP
Gene ID (NCBI) 8557
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15273
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Telethonin, also known as TCAP, is a sarcomeric protein found exclusively in striated and cardiac muscle, where it localizes to the periphery of Z discs that define the border of the sarcomere. It binds to the N-terminus of myoglobin, effectively increasing its mechanical resistance. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G(PMID: 19679566).

实验方案

Product Specific Protocols
IHC protocol for Telethonin antibody 16104-1-APDownload protocol
WB protocol for Telethonin antibody 16104-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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