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验证数据展示

  • WB analysis of human peripheral blood platelets using 67371-1-Ig (same clone as 67371-1-PBS)

    WB analysis of human peripheral blood platelets using 67371-1-Ig (same clone as 67371-1-PBS)

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67371-1-Ig (TBXAS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67371-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 67371-1-Ig (same clone as 67371-1-PBS)

    WB analysis of A549 using 67371-1-Ig (same clone as 67371-1-PBS)

    WB result of TBXAS1 antibody (67371-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBXAS1 transfected A549 cells. This data was developed using the same antibody clone with 67371-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67371-1-Ig (same clone as 67371-1-PBS)

    IHC staining of human lung cancer using 67371-1-Ig (same clone as 67371-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67371-1-Ig (TBXAS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67371-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67371-1-Ig (same clone as 67371-1-PBS)

    IHC staining of human lung cancer using 67371-1-Ig (same clone as 67371-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67371-1-Ig (TBXAS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67371-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 67371-1-Ig (same clone as 67371-1-PBS)

    FC experiment of A549 using 67371-1-Ig (same clone as 67371-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.8 ug TBXAS1 Monoclonal antibody (67371-1-Ig, Clone:1F6G6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67371-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TBXAS1 Monoclonal antibody, PBS Only

TBXAS1 Monoclonal Antibody for Indirect ELISA, IHC, WB
Cat No. 67371-1-PBS

产品说明书

CloneNo. 1F6G6

宿主/亚型

Mouse / IgG2a

种属反应性

pig, mouse, human

应用

Indirect ELISA, IHC, WB

别名

Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1, CYP5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67371-1-PBS targets TBXAS1 in Indirect ELISA, IHC, WB applications and shows reactivity with pig, mouse, human samples.

经测试应用 Indirect ELISA, IHC, WB Application Description
经测试反应性 pig, mouse, human
免疫原

CatNo: Ag29177

Product name: Recombinant human TBXAS1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 178-466 aa of BC014117

Sequence: NKNRDELNGFFNKLIRNVIALRDQQAAEERRRDFLQMVLDARHSASPMGVQDFDIVRDVFSSTGCKPNPSRQHQPSPMARPLTVDEIVGQAFIFLIAGYEIITNTLSFATYLLATNPDCQEKLLREVDVFKEKHMAPEFCSLEEGLPYLDMVIAETLRMYPPAFRFTREAAQDCEVLGQRIPAGAVLEMAVGALHHDPEHWPSPETFNPERFTAEARQQHRPFTYLPFGAGPRSCLGVRLGLLEVKLTLLHVLHKFRFQACPETQVPLQLESKSALGPKNGVYIKIVSR

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 thromboxane A synthase 1 (platelet)
别名 Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1, CYP5
计算分子量 60 kDa
观测分子量 52 kDa
GenBank蛋白编号BC014117
基因名称 TBXAS1
Gene ID (NCBI) 6916
RRIDAB_2918487
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP24557
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation.It was identified as novel airway fibroblast-specific markers with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium.It is a monomer with the molecular weight of 60 KDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669). It also reavels a molecular weight of 55 kDa in humen uterus (PMID:1386258).